Document Detail


Few effects of multi-generational dietary exposure to genistein or nonylphenol on sodium solution intake in male and female Sprague-Dawley rats.
MedLine Citation:
PMID:  19452615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous work in our laboratory indicated that lifelong dietary exposure to estrogen-like endocrine disrupters increased sodium solution intake in adult male and female rats. Here, we sought to discern the critical periods necessary for this alteration as well as establish the effects of lower dietary concentrations of genistein and nonylphenol. Male and female Sprague-Dawley rats (F0) consumed phytoestrogen-free chow containing 0, 5, 100, or 500 ppm genistein (approximately equal to 0.0, 0.4, 8.0, and 40.0 mg/kg/day) or 0, 25, 200, or 750 ppm nonylphenol (approximately equal to 0.0, 2.0, 16.0, and 60.0 mg/kg/day). Rats were mated within treatment groups and offspring (F1) maintained on the same diets. Mating for the F1, F2, and F3 (genistein only) was within treatment groups. At postnatal day (PND) 21, the F3 generation began to consume unadulterated phytoestrogen-free chow such that genistein exposure occurred only in utero and preweaning. The F4 generation was never directly exposed to genistein. On PNDs 65-68, intake of regular water and a 3.0% sodium chloride solution was measured for F1-F4 generations (genistein portion) or F1-F2 (nonylphenol portion). Although body weights were decreased by the highest dietary concentrations of genistein and nonylphenol, there were only minimal effects of exposure on sodium solution intake. As expected, intake was highest in female rats. With previous data, these results indicate that the dietary concentrations necessary to increase adult sodium solution intake in rats are greater than 500 ppm genistein and 750 ppm nonylphenol and such effects do not appear to increase across generations.
Authors:
Sherry A Ferguson; K Barry Delclos; Retha R Newbold; Katherine M Flynn
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurotoxicology and teratology     Volume:  31     ISSN:  1872-9738     ISO Abbreviation:  Neurotoxicol Teratol     Publication Date:    2009 May-Jun
Date Detail:
Created Date:  2009-05-18     Completed Date:  2009-09-16     Revised Date:  2009-10-26    
Medline Journal Info:
Nlm Unique ID:  8709538     Medline TA:  Neurotoxicol Teratol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  143-8     Citation Subset:  IM    
Affiliation:
Division of Neurotoxicology, National Center for Toxicological Research/FDA, Jefferson, AR 72079, USA. Sherry.Ferguson@fda.hhs.gov
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MeSH Terms
Descriptor/Qualifier:
Administration, Oral
Animal Feed
Animals
Body Weight
Crosses, Genetic
Diet
Female
Genistein / administration & dosage,  analysis,  pharmacology*
Isoflavones / analysis
Male
Phenols / administration & dosage,  pharmacology*
Pregnancy
Prenatal Exposure Delayed Effects
Rats
Rats, Sprague-Dawley
Sodium / administration & dosage,  metabolism*
Sodium Chloride / metabolism
Solutions
Grant Support
ID/Acronym/Agency:
IAG 224-07-007//PHS HHS
Chemical
Reg. No./Substance:
0/Isoflavones; 0/Phenols; 0/Solutions; 25154-52-3/nonylphenol; 446-72-0/Genistein; 552-66-9/daidzin; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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