Document Detail


Fetuin-A expression in early venous stenosis formation in a porcine model of hemodialysis graft failure.
MedLine Citation:
PMID:  18693047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Because fetuin-A is a cytokine with multifunctional effects on vascular smooth muscle cells and fibroblasts, the authors examined the course of its expression in early venous stenosis formation in a porcine model of chronic renal insufficiency with polytetrafluoroethylene (PTFE) arteriovenous (AV) hemodialysis grafts.
MATERIALS AND METHODS: Pigs had chronic renal insufficiency created by complete embolization of the left kidney and partial embolization of the right kidney. Twenty-eight days later, PTFE AV grafts were placed from the carotid artery to the ipsilateral jugular vein, and the animals were euthanized 3 days (n = 4), 7 days (n = 4), or 14 days (n = 4) later. Expression of fetuin-A was determined by Western blot analysis of the venous stenosis, control veins, and plasma. Immunohistochemical analysis of the venous stenosis and control vein was performed. Blood urea nitrogen (BUN) and creatinine were measured before embolization and at the time of graft placement.
RESULTS: The mean BUN and creatinine levels at graft placement were significantly higher than before embolization (P < .05). Severe venous neointimal hyperplasia occurred by day 14 and was characterized by primarily alpha-smooth muscle actin-positive cells. By day 14, fetuin-A levels had increased significantly (P < .05) at the venous stenosis compared with control veins and in the serum compared with measurements before embolization.
CONCLUSIONS: Significantly increased expression of fetuin-A was observed in early venous stenosis by day 14 and in serum compared with baseline measurements. Understanding the role of fetuin-A in venous neointimal hyperplasia could help in improving outcomes in patients undergoing hemodialysis.
Authors:
Sanjay Misra; Alex A Fu; Jill L Anderson; James F Glockner; Michael A McKusick; Haraldur Bjarnason; David A Woodrum; Debabrata Mukhopadhyay
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-08-09
Journal Detail:
Title:  Journal of vascular and interventional radiology : JVIR     Volume:  19     ISSN:  1535-7732     ISO Abbreviation:  J Vasc Interv Radiol     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-16     Completed Date:  2009-01-23     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9203369     Medline TA:  J Vasc Interv Radiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1477-82     Citation Subset:  IM    
Affiliation:
Department of Radiology, Mayo Clinic College of Medicine, 200 First Street Southwest, Alfred 6460, Rochester, MN 55905, USA. misra.sanjay@mayo.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Proteins / metabolism*
Disease Models, Animal*
Graft Occlusion, Vascular / metabolism*
Male
Swine
Venous Insufficiency / metabolism*
alpha-2-HS-Glycoprotein
Grant Support
ID/Acronym/Agency:
CA78383/CA/NCI NIH HHS; HL072178/HL/NHLBI NIH HHS; HL70567/HL/NHLBI NIH HHS; R01 CA078383-10/CA/NCI NIH HHS; R01 HL070567-08/HL/NHLBI NIH HHS; R01 HL072178-06/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/alpha-2-HS-Glycoprotein
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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