| Fetal wound healing using a genetically modified murine model: the contribution of P-selectin. | |
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MedLine Citation:
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PMID: 18405715 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: During early gestation, fetal wounds heal with paucity of inflammation and absent scar formation. P-selectin is an adhesion molecule that is important for leukocyte recruitment to injury sites. We used a murine fetal wound healing model to study the specific contribution of P-selectin to scarless wound repair. METHODS: Linear excisional wounds were created on the dorsa of E15.5 and E17.5 gestation fetuses in wild-type and P-selectin (-/-) mice (term = 19 days). Wounds were harvested at various time-points after wounding and analyzed using histology and immunohistochemistry. RESULTS: The E15.5 wounds in both wild-type and P-selectin (-/-) fetuses healed scarlessly and with minimal inflammation, whereas E17.5 wounds healed with fibrosis and inflammation. However, the scars of the P-selectin (-/-) wounds appeared slightly different than wild-type. There were significantly more inflammatory cells in E17.5 wild-type wounds 6 hours after injury (P < .001), but the difference was no longer significant by 24 hours. Finally, reepithelialization was slower in the E15.5 knockout wounds compared to their wild-type counterparts. CONCLUSIONS: Absence of P-selectin delays inflammatory cell recruitment and reepithelialization of fetal wounds; however, scar formation still occurs in late gestation wounds. The contribution of specific molecules to fetal wound healing can be elucidated using murine knockout or transgenic models. |
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Authors:
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Bindi Naik-Mathuria; Andre N Gay; Ling Yu; Jean E Hsu; C Wayne Smith; Oluyinka O Olutoye |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 43 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-04-14 Completed Date: 2008-06-13 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 675-82 Citation Subset: IM |
Affiliation:
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Division of Pediatric Surgery, Michael E. DeBakey Department of Surgery, Section of Leukocyte Biology, Baylor College of Medicine, Houston, TX 77030-2399, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Cicatrix / embryology, metabolism Fibrosis / embryology, metabolism, pathology Immunohistochemistry Inflammation / embryology, metabolism, pathology Keratinocytes / pathology Mice Mice, Inbred C57BL Mice, Knockout Models, Animal P-Selectin / metabolism* Wound Healing / physiology* Wounds and Injuries / embryology*, metabolism*, pathology |
| Grant Support | |
ID/Acronym/Agency:
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GM 069912/GM/NIGMS NIH HHS; K08 GM069912-01/GM/NIGMS NIH HHS; K08 GM069912-02/GM/NIGMS NIH HHS; K08 GM069912-03/GM/NIGMS NIH HHS; K08 GM069912-04/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/P-Selectin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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