Document Detail

Fetal wound healing using a genetically modified murine model: the contribution of P-selectin.
MedLine Citation:
PMID:  18405715     Owner:  NLM     Status:  MEDLINE    
PURPOSE: During early gestation, fetal wounds heal with paucity of inflammation and absent scar formation. P-selectin is an adhesion molecule that is important for leukocyte recruitment to injury sites. We used a murine fetal wound healing model to study the specific contribution of P-selectin to scarless wound repair.
METHODS: Linear excisional wounds were created on the dorsa of E15.5 and E17.5 gestation fetuses in wild-type and P-selectin (-/-) mice (term = 19 days). Wounds were harvested at various time-points after wounding and analyzed using histology and immunohistochemistry.
RESULTS: The E15.5 wounds in both wild-type and P-selectin (-/-) fetuses healed scarlessly and with minimal inflammation, whereas E17.5 wounds healed with fibrosis and inflammation. However, the scars of the P-selectin (-/-) wounds appeared slightly different than wild-type. There were significantly more inflammatory cells in E17.5 wild-type wounds 6 hours after injury (P < .001), but the difference was no longer significant by 24 hours. Finally, reepithelialization was slower in the E15.5 knockout wounds compared to their wild-type counterparts.
CONCLUSIONS: Absence of P-selectin delays inflammatory cell recruitment and reepithelialization of fetal wounds; however, scar formation still occurs in late gestation wounds. The contribution of specific molecules to fetal wound healing can be elucidated using murine knockout or transgenic models.
Bindi Naik-Mathuria; Andre N Gay; Ling Yu; Jean E Hsu; C Wayne Smith; Oluyinka O Olutoye
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  43     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-06-13     Revised Date:  2014-09-05    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  675-82     Citation Subset:  IM    
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MeSH Terms
Animals, Genetically Modified
Cicatrix / embryology,  metabolism
Fibrosis / embryology,  metabolism,  pathology
Inflammation / embryology,  metabolism,  pathology
Keratinocytes / pathology
Mice, Inbred C57BL
Mice, Knockout
Models, Animal
P-Selectin / metabolism*
Wound Healing / physiology*
Wounds and Injuries / embryology*,  metabolism*,  pathology
Grant Support
GM 069912/GM/NIGMS NIH HHS; K08 GM069912/GM/NIGMS NIH HHS; K08 GM069912-01/GM/NIGMS NIH HHS; K08 GM069912-02/GM/NIGMS NIH HHS; K08 GM069912-03/GM/NIGMS NIH HHS; K08 GM069912-04/GM/NIGMS NIH HHS
Reg. No./Substance:

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