| Fetal therapy with rhIGF-1 in a rabbit model of intrauterine growth retardation. | |
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MedLine Citation:
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PMID: 11421616 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Intrauterine growth retardation (IUGR) may, in part, be due to a deficiency of insulin-like growth factor-1 (IGF-1). The objectives of this study were to determine the relationship between fetal serum IGF-1 levels and fetal and placental size in a rabbit model of IUGR and to compare two techniques of selective, exogenous IGF-1 administration (transamniotic and branch uterine arterial catheter infusion) to growth-retarded fetuses in utero. MATERIALS AND METHODS: Pregnant rabbits (n = 6) had their fetuses harvested near term (31 days) for fetal and placental weighing and serum collection. Growth-retarded fetuses were selectively infused for 7 days with recombinant human IGF-1 (rhIGF-1; 1,440 ng/day) either through a transamniotic catheter (n = 8) or via an adjacent uterine arterial branch catheter (n = 6). Opposite horn runts were sham catheterized, but not infused. At term, the fetal runt pairs and their placentas were harvested and weighed, and their serum was collected. The correlation between fetal and placental weight and endogenous serum IGF-1 was calculated (Pearson coefficient, r), while paired t-tests were used to compare the means between the IGF-1-infused and control groups. RESULTS: There was a significant correlation between fetal (r = 0.4230; P = 0.022) and placental weight (r = 0.4166; P = 0.025) and endogenous serum levels of IGF-1. Transamniotic infusion of rhIGF-1 was associated with an increase in serum IGF-1 level (254 +/- 79 vs 351 +/- 101 ng/ml, P = 0.04) and placental weight (5.4 +/- 2.3 vs 7.1 +/- 3.2 g, P = 0.005), and with a trend toward increased fetal weight between matched fetal runt pairs. Fetal mortality in the uterine arterial catheterized group was 76%, and there was no significant difference in fetal or placental weight or IGF-1 levels between infused and noninfused survivors. CONCLUSIONS: Endogenous fetal serum levels correlate with fetal and placental size in the rabbit IUGR model. Transamniotic administration of rhIGF-1 significantly increases serum IGF-1 levels and placental weight of fetal runts, while uterine vessel catheterization results in prohibitive fetal mortality and does not increase fetal or placental growth or IGF-1 levels. |
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Authors:
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E D Skarsgard; L A Amii; R A Dimmitt; G Sakamoto; M E Brindle; R L Moss |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of surgical research Volume: 99 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-06-25 Completed Date: 2001-08-02 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 142-6 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Surgery, Lucile Packard Children's Hospital, Palo Alto, California 94025, USA. erik.skarsgard@medcenter.stanford.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amnion Animals Catheterization / mortality Female Fetal Blood / metabolism Fetal Death Fetal Growth Retardation / drug therapy* Humans Injections Injections, Intra-Arterial Insulin-Like Growth Factor I / administration & dosage, metabolism, therapeutic use* Organ Size / drug effects Placenta / anatomy & histology Pregnancy Rabbits Recombinant Proteins Uterus / blood supply |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 67763-96-6/Insulin-Like Growth Factor I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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