| Fetal sex determination of macaque monkeys by a nested PCR using maternal plasma. | |
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MedLine Citation:
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PMID: 20484861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Non-invasive fetal sex determination is required for biomedical studies, in which some sexual difference would be expected in fetal events, in order to make a choice of male or female fetus. To detect male fetal DNA of the sex-determining region Y gene (SRY) in maternal macaque plasma, nested real-time PCR using the SYBR Green system was developed. In all cases of pregnant macaques with male fetuses, a nested PCR product of SRY was amplified from the mother's plasma, while no amplicon was detected in any case of pregnancy with a female fetus. Interestingly, fetal SRY DNA appeared to be cleared rapidly from the maternal blood after parturition. The current method is sensitive and can be performed with a regular PCR machine. |
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Authors:
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Fusako Mitsunaga; Miyuki Ueiwa; Yoshirou Kamanaka; Mayumi Morimoto; Shin Nakamura |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental animals / Japanese Association for Laboratory Animal Science Volume: 59 ISSN: 1881-7122 ISO Abbreviation: Exp. Anim. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-05-20 Completed Date: 2010-08-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9604830 Medline TA: Exp Anim Country: Japan |
Other Details:
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Languages: eng Pagination: 255-60 Citation Subset: IM |
Affiliation:
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Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence DNA / analysis*, blood Female Genes, sry* Macaca mulatta / physiology* Male Molecular Sequence Data Polymerase Chain Reaction / methods, veterinary* Pregnancy / blood*, genetics Prenatal Diagnosis / veterinary Sequence Analysis, DNA / veterinary Sex Determination (Analysis) / methods, veterinary* |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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