| Fetal membranes act as a barrier for adenoviruses: gene transfer into exocoelomic cavity of rat fetuses does not affect cells in the fetus. | |
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MedLine Citation:
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PMID: 14749670 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: In utero gene therapy has a potential to correct genetic disorders before the first clinical symptoms appear. Our aim was to examine whether the exocoelomic cavity between amniotic and chorionic membranes offers a minimally invasive route for gene transfer to the fetus during early pregnancy. STUDY DESIGN: We injected lacZ-adenovirus (4 x 10(9) pfu) during open surgery into the exocoelomic cavity of rat fetuses (n=50) and analyzed the fetuses and rat dams for transgene expression with X-gal staining and polymerase chain reaction. RESULTS: Giant cells around Reichert's membrane, the outermost extraembryonic membrane in rodents, were transduced; but no transduction was observed in the cells of the fetuses or rat dams. CONCLUSION: In rodents, the exocoelomic cavity does not offer a route for gene transfer into the fetus. It was concluded that fetal membranes act as a barrier that prevents adenoviral particles from passing between embryonic cavities. |
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Authors:
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Anniina Laurema; Kari Vanamo; Annaleena Heikkilä; Mervi Riekkinen; Seppo Heinonen; Seppo Ylä-Herttuala |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 190 ISSN: 0002-9378 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-01-29 Completed Date: 2004-02-25 Revised Date: 2006-04-21 |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 264-7 Citation Subset: AIM; IM |
Affiliation:
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A. I. Virtanen Institute, University of Kuopio, Finland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae* Animals Chromogenic Compounds Extraembryonic Membranes* Female Fetus / physiology* Galactosides Gene Expression Gene Transfer Techniques* Genetic Vectors* Indoles Lac Operon Pregnancy Rats Rats, Wistar Staining and Labeling Transfection Transgenes |
| Chemical | |
Reg. No./Substance:
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0/Chromogenic Compounds; 0/Galactosides; 0/Indoles; 7240-90-6/5-bromo-4-chloro-3-indolyl beta-galactoside |
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