Document Detail

Fetal lung maturity assessed by fluorescence polarization: evaluation of predictive value, correction for endogenous fluorescence, and comparison with L/S ratio.
MedLine Citation:
PMID:  6821942     Owner:  NLM     Status:  MEDLINE    
The steady-state polarization (or anisotropy) of the fluorescent dye 1,6-diphenyl-1,3-5-hexatriene in amniotic fluid samples and the lecithin/sphingomyelin ratio of the samples were correlated with development of respiratory distress syndrome in newborns. We found that clinical samples have a variable endogenous fluorescence that reduces the observed polarization (or anisotropy). This background is a major interference in the assessment of fetal lung maturity by the polarization method. Correction for this interference, by also measuring the blank fluorescence and anisotropy of the sample, provides a clinical tool with a lower coefficient of variation than that of the more time-consuming lecithin/sphingomyelin ratio. The clinical correlation for 17 cases of respiratory distress syndrome in a high-risk population (60 births; twins counted as a single birth) indicates that the two methods are equivalent for predicting immature fetal lung status.
K H Cox; J B Ross; A P Peterson; T J Benedetti
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-03-17     Completed Date:  1983-03-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  346-9     Citation Subset:  IM    
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MeSH Terms
Amniotic Fluid / analysis*
Evaluation Studies as Topic
Fetal Organ Maturity
Fluorescence Polarization / methods
Infant, Newborn
Lung / embryology*
Phosphatidylcholines / analysis
Prenatal Diagnosis*
Respiratory Distress Syndrome, Newborn / diagnosis*
Sphingomyelins / analysis
Grant Support
5 F 32 CA 06589-02/CA/NCI NIH HHS
Reg. No./Substance:
0/Phosphatidylcholines; 0/Sphingomyelins; 1720-32-7/Diphenylhexatriene

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