| Fetal lung maturity assessed by fluorescence polarization: evaluation of predictive value, correction for endogenous fluorescence, and comparison with L/S ratio. | |
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MedLine Citation:
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PMID: 6821942 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The steady-state polarization (or anisotropy) of the fluorescent dye 1,6-diphenyl-1,3-5-hexatriene in amniotic fluid samples and the lecithin/sphingomyelin ratio of the samples were correlated with development of respiratory distress syndrome in newborns. We found that clinical samples have a variable endogenous fluorescence that reduces the observed polarization (or anisotropy). This background is a major interference in the assessment of fetal lung maturity by the polarization method. Correction for this interference, by also measuring the blank fluorescence and anisotropy of the sample, provides a clinical tool with a lower coefficient of variation than that of the more time-consuming lecithin/sphingomyelin ratio. The clinical correlation for 17 cases of respiratory distress syndrome in a high-risk population (60 births; twins counted as a single birth) indicates that the two methods are equivalent for predicting immature fetal lung status. |
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Authors:
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K H Cox; J B Ross; A P Peterson; T J Benedetti |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Clinical chemistry Volume: 29 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-03-17 Completed Date: 1983-03-17 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 346-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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analysis* Diphenylhexatriene Evaluation Studies as Topic Female Fetal Organ Maturity Fluorescence Polarization / methods Humans Infant, Newborn Lung / embryology* Phosphatidylcholines / analysis Pregnancy Prenatal Diagnosis* Respiratory Distress Syndrome, Newborn / diagnosis* Sphingomyelins / analysis |
| Grant Support | |
ID/Acronym/Agency:
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5 F 32 CA 06589-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylcholines; 0/Sphingomyelins; 1720-32-7/Diphenylhexatriene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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