Document Detail


Fetal distribution of 5alpha-reductase 1 and 5alpha-reductase 2, and their input on human prostate development.
MedLine Citation:
PMID:  17574609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Human prostate development starts in the tenth week of gestation. Initial interactions between the epithelium and mesenchyma are stimulated by androgens. The transformation of circulating testosterone to 5alpha-dihydrotestosterone by tissue linked 5alpha-reductase is a key event in androgen metabolism. The 5alpha-dihydrotestosterone mediates androgen effects in the urogenital sinus and external genitalia, leading to the formation of a male phenotype and androgen mediated prostate growth. Supposedly 5alpha-reductase 2 is the predominant isoenzyme in human accessory sex tissue, whereas the function of 5alpha-reductase 1 remains unclear. We focused on the detection, distribution and effects of the 2 isoenzymes during gestation and infancy. MATERIALS AND METHODS: Serial sections from fetuses and infants were immunostained using antibodies directed against 5alpha-reductase 1 and 2. Additionally, to detect the downstream products of androgen synthesis reverse transcriptase-polymerase chain reaction analyses were done for 17 beta-hydroxysteroid dehydrogenase types 2, 3 and 7. RESULTS: Immunohistochemistry revealed positive staining for each isoenzyme throughout fetal development. Moreover, reverse transcriptase-polymerase chain reaction for 5alpha-reductase 1 and 2 confirmed these findings on the transcription level. Additionally, the most relevant enzymatic downstream products of cellular androgen synthesis (17 beta-hydroxysteroid dehydrogenase 2, 3 and 7) were also detected by reverse transcriptase-polymerase chain reaction. CONCLUSIONS: To our knowledge this is the first study revealing the expression and distribution of each 5alpha-reductase isoenzyme as well as the potential contribution of 5alpha-reductase 1 during fetal human prostate development.
Authors:
A Lunacek; C Schwentner; J Oswald; H Fritsch; C Sergi; L N Thomas; R S Rittmaster; H Klocker; H Neuwirt; G Bartsch; C Radmayr
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Publication Detail:
Type:  Journal Article     Date:  2007-06-14
Journal Detail:
Title:  The Journal of urology     Volume:  178     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-13     Completed Date:  2007-08-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  716-21     Citation Subset:  AIM; IM    
Affiliation:
Department of Urology, Hanuschkrankenhaus, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
17-Hydroxysteroid Dehydrogenases / genetics
Cholestenone 5 alpha-Reductase / genetics*
Female
Gestational Age
Humans
Immunoenzyme Techniques
Infant, Newborn
Isoenzymes / genetics*
Male
Pregnancy
Prostate / embryology*,  growth & development
Reverse Transcriptase Polymerase Chain Reaction
Transcription, Genetic / genetics
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 1.1.-/17-Hydroxysteroid Dehydrogenases; EC 1.3.1.22/Cholestenone 5 alpha-Reductase

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