Document Detail


Fetal cells in maternal blood: determination of purity and yield by quantitative polymerase chain reaction.
MedLine Citation:
PMID:  7524326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The detection of fetal aneuploidy and gene mutations by analysis of fetal cells in maternal blood has demonstrated the feasibility of noninvasive prenatal diagnosis. Fetal cells are rare in the maternal circulation; all current methods used for their isolation also yield maternal cells. We developed a method that permits a quantitative assessment of the relative numbers of fetal and maternal cells. STUDY DESIGN: Samples from 40 pregnant women were flow sorted with different monoclonal antibodies. Deoxyribonucleic acid was subsequently purified from candidate fetal cells; polymerase chain reaction was performed with synthetic primers specific for sequences on chromosomes Y and 7. RESULTS: The maximum number of fetal cells detected was 52 in 1080 maternal cells. Fetal cell purity ranged from 0.001% to 4.8%. Fetal cells were detected with antibodies to CD71, CD36, and glycophorin A. CONCLUSION: Quantitative polymerase chain reaction enables the determination of the purity and yield of fetal cells remaining after isolation from maternal blood, facilitating rapid comparisons between different cell separation techniques.
Authors:
D W Bianchi; A P Shuber; M A DeMaria; A C Fougner; K W Klinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  171     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-11-15     Completed Date:  1994-11-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  922-6     Citation Subset:  AIM; IM    
Affiliation:
Department of Pediatrics, New England Medical Center, Boston, MA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / immunology
Antigens, CD / immunology
Antigens, CD36
Antigens, Differentiation, B-Lymphocyte / immunology
Cell Count
Cell Separation / methods
Female
Fetus / cytology*
Flow Cytometry
Glycophorin / immunology
Humans
Male
Polymerase Chain Reaction / methods
Pregnancy / blood*
Receptors, Transferrin
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Antigens, CD36; 0/Antigens, Differentiation, B-Lymphocyte; 0/CD71 antigen; 0/Glycophorin; 0/Receptors, Transferrin

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