Document Detail

Fetal bovine serum and other sera used in tissue culture increase epithelial permeability.
MedLine Citation:
PMID:  8463189     Owner:  NLM     Status:  MEDLINE    
Fetal bovine serum (FBS) or heat-inactivated FBS (56 degrees C for 30 min, HFBS) caused a dose-dependent decrease in the transepithelial electrical resistance of an epithelial monolayer (MDCK). A saturating concentration of HFBS (30%) caused an average fall of 25 +/- 2% within 60 min. Upon removal of HFBS, the resistance returned to its starting value within 1 h. Flux studies with [3H]mannitol demonstrate that the fall in resistance is due to an increased permeability of the tight junctions. Thirty percent heat inactivated sera from goat, newborn calf, calf, bovine, and horse caused falls ranging from 26 to 47%. In contrast with the basolateral preference of human and bovine adult sera, fetal bovine and newborn calf sera elicit this response primarily by interacting with the apical surface of the epithelium. HFBS-treated monolayers show a significant increase in the condensation of F-actin at points where > or = 3 cells meet. These results demonstrate that FBS and other sera used as nutritional supplements can increase the permeability of the tight junctions of cultured epithelial cells.
K H Mortell; A D Marmorstein; E B Cramer
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  29A     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-05-04     Completed Date:  1993-05-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  235-8     Citation Subset:  IM    
Department of Anatomy and Cell Biology, State University of New York Health Science Center, Brooklyn 11203.
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MeSH Terms
Blood Proteins / pharmacology
Culture Media / chemistry*,  pharmacology
Culture Techniques / methods
Dose-Response Relationship, Drug
Endothelial Growth Factors / analysis
Fetal Blood / chemistry*
Intercellular Junctions / drug effects
Lymphokines / analysis
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Grant Support
Reg. No./Substance:
0/Actins; 0/Blood Proteins; 0/Culture Media; 0/Endothelial Growth Factors; 0/Lymphokines; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors

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