Document Detail

Fetal blood group genotyping.
MedLine Citation:
PMID:  17593289     Owner:  NLM     Status:  MEDLINE    
Blood group genotyping using DNA extracted from fetal tissue is useful to identify fetuses at risk for hemolytic disease of the fetus and newborn (HDFN) due to maternal red cell alloantibodies. Four considerations are important for fetal blood group genotyping. First, paternal heterozygosity must be established, including tests that evaluate RHD hemizygosity. Second, the source of fetal tissue for DNA extraction requires certain considerations. Third, because the fetal genotype is used to predict the expressed phenotype, a thorough knowledge of blood group genetics is required. Moreover, the test algorithm should include the evaluation of the parental phenotypes and genotypes to help identify variant alleles. Fourth, the blood group antigen expression at birth should be evaluated to confirm the inheritance. The identification of an antigen-negative fetus on the basis of the blood group genotype provides significant advantages in managing the pregnancy at risk for HDFN. In the near future, fetal DNA in maternal plasma will likely replace fetal blood group genotyping for RHD. Significant challenges remain to detect other clinically significant blood group antigens using maternal plasma DNA.
Gregory A Denomme; Bernard J Fernandes
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Transfusion     Volume:  47     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-26     Completed Date:  2007-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  64S-8S     Citation Subset:  IM    
Research and Development, Canadian Blood Services, Toronto, Canada.
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MeSH Terms
Blood Group Antigens / analysis*,  genetics
Blood Specimen Collection / methods
DNA / isolation & purification
Erythroblastosis, Fetal / diagnosis
Fetal Blood / chemistry*
Inheritance Patterns
Molecular Diagnostic Techniques / methods*
Prenatal Diagnosis
Reg. No./Substance:
0/Blood Group Antigens; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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