Document Detail


Fetal blood group genotyping.
MedLine Citation:
PMID:  17593289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Blood group genotyping using DNA extracted from fetal tissue is useful to identify fetuses at risk for hemolytic disease of the fetus and newborn (HDFN) due to maternal red cell alloantibodies. Four considerations are important for fetal blood group genotyping. First, paternal heterozygosity must be established, including tests that evaluate RHD hemizygosity. Second, the source of fetal tissue for DNA extraction requires certain considerations. Third, because the fetal genotype is used to predict the expressed phenotype, a thorough knowledge of blood group genetics is required. Moreover, the test algorithm should include the evaluation of the parental phenotypes and genotypes to help identify variant alleles. Fourth, the blood group antigen expression at birth should be evaluated to confirm the inheritance. The identification of an antigen-negative fetus on the basis of the blood group genotype provides significant advantages in managing the pregnancy at risk for HDFN. In the near future, fetal DNA in maternal plasma will likely replace fetal blood group genotyping for RHD. Significant challenges remain to detect other clinically significant blood group antigens using maternal plasma DNA.
Authors:
Gregory A Denomme; Bernard J Fernandes
Related Documents :
18448739 - Bilirubin/albumin ratios in fetal blood and in amniotic fluid in rhesus immunization.
3733629 - Influence of upper respiratory tract on liquid flow to and from fetal lungs.
6205389 - Changes in maternal serum alpha-fetoprotein levels associated with intrauterine genetic...
6525209 - Effect of cephalic pressure on fetal cerebral blood flow.
7394719 - Absorption of methylmercury by the fetal guinea pig during mid to late gestation.
2696779 - Intrauterine treatment of severe fetal erythroblastosis: intravascular transfusion with...
11552299 - Justification of measurement of eight congeners levels instead of twenty congeners of d...
6487689 - Modulation of the blood-brain barrier permeability in neonatal cytotoxic brain edema: l...
1489499 - A quantitative assessment of blood loss in burn wound excision and grafting.
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Transfusion     Volume:  47     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-26     Completed Date:  2007-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  64S-8S     Citation Subset:  IM    
Affiliation:
Research and Development, Canadian Blood Services, Toronto, Canada. greg.denomme@blood.ca
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alleles
Blood Group Antigens / analysis*,  genetics
Blood Specimen Collection / methods
DNA / isolation & purification
Erythroblastosis, Fetal / diagnosis
Female
Fetal Blood / chemistry*
Genotype
Humans
Inheritance Patterns
Molecular Diagnostic Techniques / methods*
Paternity
Pregnancy
Prenatal Diagnosis
Chemical
Reg. No./Substance:
0/Blood Group Antigens; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular genetic blood group typing by the use of PCR-SSP technique.
Next Document:  Effects of the remote C fibres stimulation induced by capsaicin on the blink reflex in chronic migra...