Document Detail

Fetal and adult localization of C/EBP: evidence for combinatorial action of transcription factors in cell-specific gene expression.
MedLine Citation:
PMID:  2401206     Owner:  NLM     Status:  MEDLINE    
C/EBP is a positive-acting transcription factor important for hepatocyte-specific expression present not only in hepatocytes but also a limited number of other cell types in adult mice. By Northern blot analysis and in situ hybridization experiments with mouse embryos and adult tissues, we first detected C/EBP mRNA in hepatocytes at the 13th gestational day, when no other cell types give detectable signals and thus by this test C/EBP is, at least in the embryo, a 'liver-specific' factor. Only trace amounts of C/EBP were seen in the yolk sac and no mRNA was detectable in choroid plexus in either embryos or adult animals. Both these cell types produce some proteins (e.g. albumin, transthyretin, alpha-1 antitrypsin and others) that are also made in the liver where C/EBP is important for their production; thus either fewer factors or different factors govern yolk sac and choroid plexus production of these proteins. C/EBP mRNA was not detected in fetal brain but was present in several regions of the adult mouse brain again emphasizing that this factor does not appear to have a very early embryologic role. In the adult brain, it was most concentrated in CA1 to CA4 regions of the hippocampus, in cerebellar Purkinje cells, and in layer II and III of the cortex.
C F Kuo; K G Xanthopoulos; J E Darnell
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  109     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1990 Jun 
Date Detail:
Created Date:  1990-10-24     Completed Date:  1990-10-24     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  473-81     Citation Subset:  IM    
Laboratory of Molecular Cell Biology, Rockefeller University, New York 10021.
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MeSH Terms
Blotting, Northern
Brain Chemistry
Gene Expression / genetics*
Liver / cytology,  embryology,  physiology*
Mice, Inbred Strains
Nucleic Acid Hybridization
RNA, Messenger / analysis
Transcription Factors / analysis,  genetics*
Reg. No./Substance:
0/RNA, Messenger; 0/Transcription Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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