Document Detail

Fetal DNA clearance from maternal plasma is impaired in preeclampsia.
MedLine Citation:
PMID:  12446469     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Increased fetal DNA in maternal plasma/serum has been reported in pregnancies complicated by preeclampsia. We hypothesized that impaired clearance of fetal DNA might contribute, at least in part, to the above-mentioned phenomenon. METHODS: We studied 7 preeclamptic and 10 control pregnant women. All had male fetuses. Serial blood samples were obtained from before delivery to 6 h postpartum. Male fetal DNA in maternal plasma was measured by real-time quantitative PCR for the SRY gene on the Y chromosome. RESULTS: The median fetal DNA concentrations before delivery were significantly higher in the preeclamptic women than in the controls (521 vs 227 genome-equivalents/mL for preeclamptic and control women, respectively; Mann-Whitney rank-sum test, P = 0.017). The median fetal DNA concentrations at 6 h after delivery were also significantly different between the two groups (208 vs 0 genome-equivalents/mL for preeclamptic and control women, respectively; Mann-Whitney rank-sum test, P = 0.002). A first-order clearance model was found to best describe the kinetics of maternal plasma fetal DNA clearance. Moreover, we observed a significant difference in the median apparent clearance half-lives of fetal DNA between the preeclamptic women (114 min) and controls (28 min; Mann-Whitney rank-sum test, P = 0.007). CONCLUSIONS: This study represents the first documentation of impaired fetal DNA clearance from maternal plasma in preeclampsia. Such an abnormality in circulating DNA clearance may also be present in other medical conditions associated with quantitative aberrations in circulating DNA concentrations.
Tai-Wah Lau; Tse N Leung; Lisa Y S Chan; Tze K Lau; K C Allen Chan; Wing H Tam; Y M Dennis Lo
Related Documents :
25119709 - Analysis of the dna interaction of copper compounds belonging to the casiopeínas® antit...
22908299 - Dual recognition of phosphoserine and phosphotyrosine in histone variant h2a.x by dna d...
17852189 - Toxoplasma gondii detection by nested polymerase chain reaction in lens aspirate and pe...
22648529 - An in vitro study on the dna damaging effects of phytochemicals partially isolated from...
10830749 - Lack of integrated tt virus (ttv) genomes in cellular dna in infected human hematopoiet...
22607419 - Dna and rna folds in transcription complex as evidenced by iodine-125 radioprobing.
6253659 - Identification of the avian myeloblastosis virus genome. ii. restriction endonuclease a...
1890169 - Detection of helicobacter pylori by using the polymerase chain reaction.
9118229 - Mobility of yeast mitochondrial group ii introns: engineering a new site specificity an...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  48     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-26     Completed Date:  2002-12-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2141-6     Citation Subset:  IM    
Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
DNA / blood*
Polymerase Chain Reaction
Pre-Eclampsia / blood*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Genotyping of essential hypertension single-nucleotide polymorphisms by a homogeneous PCR method wit...
Next Document:  Hepatitis C genotype determination by melting curve analysis with a single set of fluorescence reson...