Document Detail


Fetal CD34+ cells in the maternal circulation and long-term microchimerism in rhesus monkeys (Macaca mulatta).
MedLine Citation:
PMID:  15665761     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Studies in humans have shown that during pregnancy fetal cells can enter the maternal circulation and persist for many years. While we have previously reported the presence of cell-free fetal DNA during pregnancy in rhesus monkeys, it is unknown whether cells circulate and persist long term in maternal tissues. In this study, we asked whether fetal CD34 cells can be found in the maternal circulation and if male fetal cells persist in maternal tissues postdelivery. METHODS: The presence of the Y chromosome in maternal blood and tissues was assessed using real-time PCR assays for the sex determining region Y (SRY) and testes specific protein Y (TSPY) genes. Analysis was done on CD34 and CD34 cells isolated from maternal blood collected at select time points during gestation from gravid animals with male or female fetuses, and tissues were analyzed from nongravid animals that had previously delivered male offspring. RESULTS: All animals with male fetuses tested positive for the Y chromosome in CD34 cells (0-30 cells/50,000 genome equivalents). Y sequences were also found in one or more maternal tissues collected up to 3-years postdelivery (thyroid, heart, spleen, liver, pituitary, adrenals, skin, inguinal lymph nodes). CONCLUSION: These studies suggest transfer of fetal CD34 cells during pregnancy and persistent fetal microchimerism in the rhesus model. Thus, rhesus monkeys can be used to further our understanding of fetal:maternal microchimerism and the role of fetal cells in maternal health and disease.
Authors:
Daniel F Jimenez; Alyssa C Leapley; Chang I Lee; Man-Ni Ultsch; Alice F Tarantal
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Transplantation     Volume:  79     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-24     Completed Date:  2005-03-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  142-6     Citation Subset:  IM    
Affiliation:
California National Primate Research Center, University of California, Pedrick and Hutchison Roads, Davis, CA 95616-8542, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD34 / blood*
Chimera / immunology*
Female
Fetal Blood / cytology*,  immunology
Gestational Age
Macaca mulatta
Male
Maternal-Fetal Exchange*
Polymerase Chain Reaction
Pregnancy
Sex Determination (Genetics)
Y Chromosome
Grant Support
ID/Acronym/Agency:
HL53762/HL/NHLBI NIH HHS; HL69748/HL/NHLBI NIH HHS; RR00169/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD34

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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