| Fertilization in a chiton: acrosome-mediated sperm-egg fusion. | |
| | |
MedLine Citation:
|
PMID: 3246365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Contrary to the widely accepted view that chiton sperm lack acrosomes and that fertilization in this group occurs via a micropyle, we demonstrate here that fertilization in Tonicella lineata occurs by acrosome-mediated sperm-egg fusion. The acrosome is a small vesicle containing two granules located at the tip of the sperm. The eggs have an elaborate hull (= chorion), which is formed into cupules that remain covered by follicle cells until maturity. When dissected ripe eggs were exposed to sperm in vitro, the sperm were attracted only to open cupules, inside which they swam through one of seven channels to the base where they penetrated the hull. The acrosome fired on contact with, or in, the hull, and during passage through it the apical granule was exhausted while the basal granule was exposed. If sperm contacted follicle cells between the cupules the acrosome did not react. The vitelline layer beneath the hull contains pores arranged in a regular pattern. Embedded in the base of each pore is an egg microvillus. Having penetrated the hull the sperm anterior filament located a pore and fused with the tip of the egg microvillus projecting into it. This created a membranous tube, through which the sperm nucleus was injected into the egg. The egg membrane appeared to be raised up into a small fertilization cone around the penetrating sperm, the vitelline layer became slightly elevated, and some cortical granules were released by exocytosis. |
| | |
Authors:
|
J Buckland-Nicks; R Koss; F S Chia |
Related Documents
:
|
1920285 - Effect of ovulation on sperm transport in the hamster oviduct. 12762415 - Does preejaculatory penile secretion originating from cowper's gland contain sperm? 11287015 - Effect of the total motile sperm count on the efficacy and cost-effectiveness of intrau... 9379125 - In vitro fertilizing capacity of sperm from fsh-treated photoinhibited djungarian hamst... 2762235 - Trisomy 12 mosaicism in amniocytes and dysmorphic child despite normal chromosomes in f... 6422765 - Fetal pulmonary maturity in isoimmunized pregnancies. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Gamete research Volume: 21 ISSN: 0148-7280 ISO Abbreviation: Gamete Res Publication Date: 1988 Nov |
Date Detail:
|
Created Date: 1989-06-16 Completed Date: 1989-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7806559 Medline TA: Gamete Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 199-212 Citation Subset: IM |
Affiliation:
|
Department of Zoology, University of Alberta, Edmonton, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acrosome
/
enzymology,
physiology,
ultrastructure Animals Female Fertilization Male Microvilli / physiology Models, Anatomic Mollusca / physiology* Organelles / physiology Ovum / ultrastructure Sperm-Ovum Interactions Spermatozoa / ultrastructure |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Sequence analysis of a processed gene coding for mouse ribosomal protein L32.
Next Document: Wheat germ agglutinin treatment of follicle cell-free mouse oocytes inhibits fertilization.