Document Detail


Fertilization, embryonic development and implantation of mouse oocytes with one or two laser-drilled holes in the zona, and inseminated at different sperm concentrations.
MedLine Citation:
PMID:  8671439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The zonae pellucidae of mouse oocytes were photo-ablated by an ultraviolet laser (337.1 nm) to create one or two 10 microm holes. The pulse energy used was approximately 3 microJ/s, with a frequency of 10 pulse/s. These laser zona-drilled (LZD) oocytes with one hole (LZD1) or two holes (LZD2) and the zona-intact controls were inseminated with spermatozoa at standard concentrations of 5x10(4), 5x10(5), 1x10(6) and 2x10(6)/ml. Fertilization was significantly improved at all sperm concentrations in LZD1 and LZD2 oocytes as compared to the controls. However, there was no significant difference in fertilization rates between LZD1 and LZD2. LZD2 produced significantly higher numbers of blastocysts at day 5. Hatching was significantly enhanced in the presence of either one or two holes in the zona. Polyploidy was generally absent, except in LZD2 oocytes (1%) inseminated at higher sperm concentrations. Differential cell counts of expanded LZD blastocysts were similar to those of the controls. Significantly fewer LZD2 blastocysts implanted and produced viable fetuses than LZD1 and control blastocysts. Morphological abnormalities of the fetuses were absent in all three groups. Laser zona-drilling using the ultraviolet laser was shown to be fast, efficient and safe.
Authors:
S L Liow; A Bongso; S C Ng
Related Documents :
18081939 - Polycystic ovary syndrome and oocyte developmental competence.
16728089 - In vitro and in vivo developmental capacity of oocytes from prepubertal and adult sheep.
12217319 - An essential role for functional telomeres in mouse germ cells during fertilization and...
15749489 - Intracytoplasmic sperm injection as a treatment for unexplained total fertilization fai...
2959179 - Oocyte retrieval for in vitro fertilization.
1895329 - The macaque model for in vitro fertilization: superovulation techniques and ultrasound-...
356409 - The effect of gn-rh on the pregnancy rate of artificially inseminated cows.
18172239 - Clinical decision making in restorative dentistry, endodontics, and antibiotic prescrip...
7252919 - Protein, prostaglandin and steroid synthesis in caruncular and intercaruncular endometr...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  11     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-10-10     Completed Date:  1996-10-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1273-80     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, National University of Singapore, Lower Kent Ridge Road, Singapore 119074, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Embryonic and Fetal Development
Female
Fertilization in Vitro / methods*
Lasers*
Male
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Oocytes*
Pregnancy
Sperm Count
Ultraviolet Rays
Zona Pellucida / ultrastructure*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cryopreservation of human ovarian tissue using dimethylsulphoxide and propanediol-sucrose as cryopro...
Next Document:  Insulin-like growth factor-binding proteins produced by Vero cells, human oviductal cells and human ...