Document Detail


Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in winter.
MedLine Citation:
PMID:  12487447     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two experiments in two seasons evaluated fertilization rate and embryonic development in dairy cattle. Experiment 1 (summer) compared lactating Holstein cows (n = 27; 97.3 +/- 4.1 d postpartum [dppl; 40.0 +/- 1.5 kg milk/d) to nulliparous heifers (n = 28; 11 to 17 mo old). Experiment 2 (winter) compared lactating cows (n = 27; 46.4 +/- 1.6 dpp; 45.9 +/- 1.4 kg milk/d) to dry cows (n = 26). Inseminations based on estrus included combined semen from four high-fertility bulls. Embryos and oocytes recovered 5 d after ovulation were evaluated for fertilization, embryo quality (1 = excellent to 5 = degenerate), nuclei/embryo, and accessory sperm. In experiment 1, 21 embryos and 17 unfertilized oocytes (UFO) were recovered from lactating cows versus 32 embryos and no UFO from heifers (55% vs. 100% fertilization). Embryos from lactating cows had inferior quality scores (3.8 +/- 0.4 vs. 2.2 +/- 0.3), fewer nuclei/embryo (19.3 +/- 3.7 vs. 36.8 +/- 3.0) but more accessory sperm (37.3 +/- 5.8 vs. 22.4 +/- 5.5/embryo) than embryos from heifers. Sperm were attached to 80% of UFO (17.8 +/- 12.1 sperm/UFO). In experiment 2, lactating cows yielded 36 embryos and 5 UFO versus 34 embryos and 4 UFO from dry cows (87.8 vs. 89.5% fertilization). Embryo quality from lactating cows was inferior to dry cows (3.1 +/- 0.3 vs. 2.2 +/- 0.3), but embryos had similar numbers of nuclei (27.2 +/- 2.7 vs. 30.6 +/- 2.1) and accessory sperm (42.0 +/- 9.4 vs. 36.5 +/- 6.3). From 53% of the flushings from lactating cows and 28% from dry cows, only nonviable embryos were collected. Thus, embryos of lactating dairy cows were detectably inferior to embryos from nonlactating females as early as 5 d after ovulation, with a surprisingly high percentage of nonviable embryos. In addition, fertilization rate was reduced only in summer, apparently due to an effect of heat stress on the oocyte.
Authors:
R Sartori; R Sartor-Bergfelt; S A Mertens; J N Guenther; J J Parrish; M C Wiltbank
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of dairy science     Volume:  85     ISSN:  0022-0302     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-12-18     Completed Date:  2003-04-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2803-12     Citation Subset:  IM    
Affiliation:
Department of Dairy Science, University of Wisconsin, Madison 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle / embryology*,  physiology*
Embryonic and Fetal Development*
Female
Insemination, Artificial / veterinary
Lactation / physiology*
Male
Oocytes / growth & development,  physiology
Parity
Pregnancy
Pregnancy Rate
Seasons
Sperm-Ovum Interactions / physiology*
Spermatozoa / physiology
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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