Document Detail

Fertilizability, developmental competence, and chromosomal integrity of oocytes microinjected with pre-treated spermatozoa in mice.
MedLine Citation:
PMID:  19955207     Owner:  NLM     Status:  MEDLINE    
The aim of the present study was to investigate the safety of sperm pre-treatment during the ICSI procedure using a mouse model. Mouse spermatozoa were treated with methyl-beta-cyclodextrin, lysolecithin, Triton X-100, and dithiothreitol (DTT), and injected into mouse oocytes. The injected oocytes were monitored for chromosomal integrity and pre- and post-implantation development. The chromosomal integrity of the injected oocytes was impaired by in vitro incubation and chemical antagonism. Particularly in the 60-min DTT group, severe chromosome damage increased. Despite the chromosomal damage, the resultant embryos frequently developed to the blastocyst stage. However, the embryos in the 60-min DTT group had significantly higher chromosomal damage and decreased developmental competence to live fetuses. These results indicate that excessive sperm pre-treatment such as DTT for 60 min generates severe chromosome damage in injected oocytes, and that the damage decreases developmental competence to live fetuses but not to blastocysts.
Hiroyuki Watanabe; Hiroshi Suzuki; Yutaka Fukui
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-12-02
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  139     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-19     Completed Date:  2010-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  513-21     Citation Subset:  IM    
Department of Food Production Science, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
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MeSH Terms
Cells, Cultured
Chromosomes / drug effects,  metabolism*
DNA Fragmentation / drug effects
Dithiothreitol / pharmacology
Embryonic Development* / drug effects,  physiology
Fertilization in Vitro* / drug effects,  methods
Lysophosphatidylcholines / pharmacology
Mice, Inbred C57BL
Mice, Inbred ICR
Octoxynol / pharmacology
Oocytes / drug effects,  physiology
Specimen Handling / methods
Sperm Capacitation / drug effects,  physiology
Sperm Injections, Intracytoplasmic / methods*
Spermatozoa / drug effects,  physiology*
Time Factors
beta-Cyclodextrins / pharmacology
Reg. No./Substance:
0/Lysophosphatidylcholines; 0/beta-Cyclodextrins; 0/methyl-beta-cyclodextrin; 3483-12-3/Dithiothreitol; 9002-93-1/Octoxynol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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