Document Detail


Fertility of superovulated ewes following intrauterine or oviducal insemination with fresh or frozen-thawed semen.
MedLine Citation:
PMID:  1957009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two experiments were conducted with mature Merino ewes to investigate the effects of time and site of insemination of fresh and frozen-thawed semen on the fertility of superovulated ewes. In Experiment 1, each ewe was treated with an intravaginal progestagen sponge and PMSG and/or FSH. They were inseminated in the uterus with fresh or frozen-thawed semen (approximately 100 x 10(6) motile sperm) at 24, 44 or 64 h after sponge withdrawal. Ova were recovered at 88 h after sponge withdrawal and classified as fertilized if they had pronuclei or had cleaved. Mean fertilization rates of recovered ova were 60.0, 93.7 and 87.8% for fresh semen and 46.1, 98.2 and 26.1% for frozen-thawed semen at each of the insemination times (24, 44 and 64 h) respectively. Overall, fertilization rates were higher for fresh semen than for frozen-thawed semen (P less than 0.01), but there was an interaction with time of insemination (P less than 0.01). Following insemination with frozen-thawed semen at 64 h, only 61% of the fertilized ova developed to the 2- to 4-cell stage by the time of embryo recovery; this was less than in any of the other groups (P less than 0.05). In Experiment 2, ewes were inseminated with 100 x 10(6) motile fresh or frozen-thawed semen in the uterus or in the oviducts at 64 h after sponge withdrawal.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
H N Jabbour; G Evans
Related Documents :
428579 - The value of hysterosalpingography prior to donor artificial insemination.
22535759 - Maternal nutritional status in early pregnancy is associated with body water and plasma...
569209 - The jelly envelopes and fertilization of eggs of the newt, notophthalmus viridescens.
17681679 - Efficiency of short-term storage of equine semen in a simple-design cooling system.
16726769 - Effect of dialysis or centrifugation on post-thaw motility and fertility of santa gertr...
22458909 - Alcohol prevention in swedish antenatal care: effectiveness and perceptions of the risk...
105529 - Suppression of uterine motor activity by prostaglandin synthetase inhibitor naproxen.
12746989 - Three-dimensional yolk and gestational sac volume. a prospective study of prognostic va...
21714679 - Glycemic control and pregnancy outcomes in women with type 1 diabetes mellitus using li...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  3     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  1991  
Date Detail:
Created Date:  1991-12-31     Completed Date:  1991-12-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  1-7     Citation Subset:  IM    
Affiliation:
Department of Animal Science, University of Sydney, NSW, Australia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cryopreservation
Female
Fertility*
Insemination, Artificial / methods,  veterinary*
Male
Oviducts
Pregnancy
Semen
Sheep
Superovulation
Uterus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  In situ tumour radiosensitization induced by clofibrate administration: single dose and fractionated...
Next Document:  Effect of lupin (Lupinus angustifolius) supplementation on ovarian and pituitary activity in ewes.