Document Detail

Fertility of mice receiving vitrified adult mouse ovaries.
MedLine Citation:
PMID:  16595719     Owner:  NLM     Status:  MEDLINE    
Cryopreservation of the ovaries is a useful technology for preservation of germ cells from experimental animals, because if the female founder is infertile or has mutated mitochondrial DNA, preservation of female germ cells is necessary. Although it is possible to cryopreserve immature mouse ovaries with a high degree of viability by vitrification with a mixture of several cryoprotectants, the viability of cryopreserved adult mouse ovaries is still unknown. Here, we investigated the viability of mouse ovaries at various ages after cryopreservation by vitrification techniques. Donor ovaries were collected from 10-day-, 4-week-, 10-week- and 7-month-old, female, nulliparous, green fluorescence protein (GFP)-transgenic mice and cryopreserved by vitrification. The vitrified-warmed ovaries were orthotopically transplanted to 4- or 10-week-old mice. GFP-positive pups were obtained in all experimental groups. In the 4-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week- and 7-month-old donors were 44%, 9%, 12% and 4% respectively. In the 10-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week- and 7-month-old donors were 36%, 16%, 2% and 9% respectively. Furthermore, GFP-positive pups also were obtained from recipients transplanted with ovaries of donors without normal estrous cyclicity. Our results indicate that cryopreservation of mouse ovaries by vitrification is a useful method for the preservation of female germ cells from mice of various ages.
Toshio Hani; Takanori Tachibe; Saburo Shingai; Nobuo Kamada; Otoya Ueda; Kou-ichi Jishage
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  131     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-05     Completed Date:  2006-10-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  681-7     Citation Subset:  IM    
Chugai Research Institute for Medical Science, Inc., Gotemba, Shizuoka, 412-8513 Japan.
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MeSH Terms
Cryopreservation / methods*
Green Fluorescent Proteins / genetics
Litter Size
Mice, Inbred C57BL
Mice, Transgenic
Oocytes / physiology
Ovary / transplantation*
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins

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