| Fertility after post-cervical artificial insemination with cryopreserved sperm from boar ejaculates of good and poor freezability. | |
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MedLine Citation:
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PMID: 19577868 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study compared the field fertility outcomes in frozen-thawed (FT) sperm from boar ejaculates with different freezability (good, GFE/poor, PFE) while testing the reliability of the post-cervical artificial insemination (post-CAI) in FT sperm. The assay was conducted over eight months with 86 weaned sows being inseminated by post-CAI. Every ejaculate in a total of 26 from 15 Pi?train boars was divided into a refrigerated semen portion (FS; control treatment) and a cryopreserved portion (FT sperm), and the ejaculates were in turn classified as GFE or PFE in function of the sperm progressive motility and viability at 240min post-thaw. As result, one of four possible treatments was randomly given to each sow: FS-GFE, FS-PFE, FT-GFE and FT-PFE. The number of pregnant and farrowing sows in FT-GFE did not significantly differ from those of FS control treatments. Contrarily, the probabilities of pregnancy were two times lower after inseminations with FT-PFE (P<0.05) compared to FT-GFE, which indicates that ejaculates with high post-thaw sperm progressive motility and viability are more likely to result in pregnancies than those with poor in vitro sperm function. There were no differences in litter size or the risk of backflow among treatments. Further trials are required to determine the optimal volume and concentration of FT sperm in post-CAI to obtain a more reliable method for farmers interested in cryopreserved sperm. |
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Authors:
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I Casas; S Sancho; M Briz; E Pinart; E Bussalleu; M Yeste; S Bonet |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Animal reproduction science Volume: 118 ISSN: 1873-2232 ISO Abbreviation: Anim. Reprod. Sci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2009-12-16 Completed Date: 2010-03-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7807205 Medline TA: Anim Reprod Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 69-76 Citation Subset: IM |
Affiliation:
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Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Campus Montilivi s/n, 17071 Girona, Spain. isabel.casas@udg.edu |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Cervix Uteri Cryopreservation* / veterinary Ejaculation / physiology Female Fertility / physiology* Freezing / adverse effects Insemination, Artificial / methods*, veterinary Litter Size Male Pregnancy Pregnancy Rate Semen / cytology, physiology Semen Analysis Semen Preservation / adverse effects, methods*, veterinary Swine* / physiology |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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