| Ferromagnetic micropallets for magnetic capture of single adherent cells. | |
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MedLine Citation:
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PMID: 20968293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present a magnetic micropallet array and demonstrate its capacity to recover specific, individual adherent cells from large populations and deliver them for downstream single cell analysis. A ferromagnetic photopolymer was formulated, characterized, and used to fabricate magnetic micropallets, which are microscale pedestals that provide demarcated cell growth surfaces with preservation of biophysical properties including photopatternability, biocompatibility, and optical clarity. Each micropallet holds a single adherent cell in culture, and hundreds of thousands of micropallets comprise a single micropallet array. Any micropallet in the array can be recovered on demand, carrying the adhered cell with it. We used this platform to recover selectively single cells, which were subsequently analyzed using single-cell RT-qPCR. |
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Authors:
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Nicholas M Gunn; Ruth Chang; Trisha Westerhof; Guann-Pyng Li; Mark Bachman; Edward L Nelson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-22 |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: 26 ISSN: 1520-5827 ISO Abbreviation: Langmuir Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-10 Completed Date: 2011-03-04 Revised Date: 2011-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
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Languages: eng Pagination: 17703-11 Citation Subset: IM |
Affiliation:
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School of Engineering, Department of Biomedical Engineering, University of California at Irvine, Irvine, California 92697, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biophysical Processes Cell Adhesion Extracellular Matrix / metabolism Ferric Compounds / chemistry*, metabolism*, toxicity Fluorescent Antibody Technique Humans Light Magnetics* Materials Testing Mice Microtechnology / instrumentation* Molecular Imaging NIH 3T3 Cells Nanoparticles / chemistry* Polymerase Chain Reaction Rats Single-Cell Analysis |
| Grant Support | |
ID/Acronym/Agency:
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CA132039/CA/NCI NIH HHS; P41 RR001192-32/RR/NCRR NIH HHS; P41RR001192/RR/NCRR NIH HHS; R21 CA132039-02/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ferric Compounds; 1309-37-1/ferric oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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