| Ferrocenyl glycopeptides as electrochemical probes to detect autoantibodies in multiple sclerosis patients' sera. | |
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MedLine Citation:
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PMID: 18273890 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycopeptide analogues of CSF114(Glc), modified at N-terminus with new ferrocenyl carboxylic acid and a new ferrocenyl-thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these "electrochemical probes" did not affect autoantibody recognition both in SP-ELISA and in inhibition experiments. By electrochemical monitoring the interactions of the modified peptides Fc-CSF114(Glc) and 4-FcPhP(S)Abu-CSF114(Glc) with the autoantibodies, we demonstrated that autoantibodies could be detected with a sensitivity comparable to ELISA method. The new electrochemical probes can be proposed to characterize autoantibodies as biomarkers of multiple sclerosis by a simple, rapid, and reproducible cyclic voltammetry-based diagnostic methodology. |
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Authors:
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Feliciana Real-Fernández; Amélie Colson; Jérome Bayardon; Francesca Nuti; Elisa Peroni; Rita Meunier-Prest; Francesco Lolli; Mario Chelli; Christophe Darcel; Sylvain Jugé; Anna Maria Papini |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biopolymers Volume: 90 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2008 |
Date Detail:
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Created Date: 2008-06-23 Completed Date: 2009-01-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 488-95 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2008 Wiley Periodicals, Inc. |
Affiliation:
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Laboratory of Peptide and Protein Chemistry and Biology, Polo Scientifico e Tecnologico, Department of Organic Chemistry Ugo Schiff and CNR ICCOM, University of Florence, and Azienda Ospedaliera Careggi, Firenze, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens Autoantibodies / blood* Chromatography, Liquid Electrochemistry Ferrous Compounds / chemistry, metabolism* Glycopeptides / chemical synthesis, chemistry, metabolism* Gold Humans Immunoenzyme Techniques Molecular Probes / metabolism* Multiple Sclerosis / blood* Solutions |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Autoantibodies; 0/Ferrous Compounds; 0/Glycopeptides; 0/Molecular Probes; 0/Solutions; 102-54-5/ferrocene; 7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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