Document Detail

Ferritin expression in rat hepatocytes and Kupffer cells after lead nitrate treatment.
MedLine Citation:
PMID:  19332663     Owner:  NLM     Status:  MEDLINE    
Lead nitrate induces hepatocyte proliferation and subsequent apoptosis in rat livers. Iron is a constituent of heme and is also required for cell proliferation. In this study, the expression of ferritin light-chain (FTL), the major iron storage protein, was investigated in rat livers after a single intravenous injection of lead nitrate. Western blotting and immunohistochemistry revealed that FTL was increased in hepatocytes around the central veins and strongly expressed in nonparenchymal cells. Some FTL-positive nonparenchymal cells were identified as Kupffer cells that were positive for CD68. FTL-positive Kupffer cells occupied about 60% of CD68-positive cells in the periportal and perivenous areas. The relationships between FTL expression and apoptosis induction or the engulfment of apoptotic cells were examined. TUNEL-positive cells were increased in the treatment group, and enhanced expression of milk fat globule EGF-like 8 was demonstrated in some Kupffer cells and hepatocytes, indicating enhanced apoptosis induction and phagocytosis of apoptotic cells. FTL-positive Kupffer cells were not detected without lead nitrate treatment or in rat livers treated with clofibrate, which induces hepatocyte proliferation but not apoptosis. These results suggest that FTL expression in Kupffer cells after lead treatment is dependent on phagocytosis of apoptotic cells.
Yang Fan; Toshiyuki Yamada; Takeshi Shimizu; Naoki Nanashima; Miki Akita; Kohji Suto; Shigeki Tsuchida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicologic pathology     Volume:  37     ISSN:  1533-1601     ISO Abbreviation:  Toxicol Pathol     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-03-31     Completed Date:  2009-06-16     Revised Date:  2012-01-05    
Medline Journal Info:
Nlm Unique ID:  7905907     Medline TA:  Toxicol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  209-17     Citation Subset:  IM    
Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
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MeSH Terms
Antigens, CD / metabolism
Apoptosis / drug effects
Cell Proliferation / drug effects
Ferritins / genetics,  metabolism*
Gene Expression / drug effects*
Hepatocytes / metabolism*
In Situ Nick-End Labeling
Kupffer Cells / metabolism*
Lead / pharmacology*,  toxicity
Nitrates / pharmacology*,  toxicity
Phagocytosis / drug effects
Rats, Sprague-Dawley
Reg. No./Substance:
0/Antigens, CD; 0/Nitrates; 18256-98-9/lead nitrate; 7439-92-1/Lead; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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