Document Detail

Ferritin enhances SPIO tracking of C6 rat glioma cells by MRI.
MedLine Citation:
PMID:  20440566     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To investigate the effect of ferritin protein overexpression on superparamagnetic iron oxide (SPIO) particle labeling of C6 rat glioma cells, and track the labeled cells in vivo using magnetic resonance imaging (MRI).
MATERIALS AND METHODS: A plasmid of H-chain of murine ferritin gene was constructed and transfected into C6 cells. The parental and the transfected C6 cells labeled with SPIO were bilaterally inoculated subcutaneously into nude mice. The mice were imaged by multiple T2-weighted MR scans after C6 cell inoculation. The mice were killed 2 weeks later, and the concentration of iron in the tumor tissue was measured by inductively coupled plasma.
RESULTS: The iron concentration in xenografts derived from SPIO-labeled C6 cells that were transfected with ferritin plasmid was significantly higher than that in xenografts from parental C6 cells that were labeled with SPIO but not transfected (p = 0.034, N = 5). Ferritin-transfected C6 cells showed an improved T(2) contrast in vivo compared with parental cells labeled with SPIO but not transfected.
CONCLUSION: Coordinating ferritin with SPIO can lead to a longer MRI cellular tracking period.
Jiandong Wang; Jin Xie; Xiaojun Zhou; Zhen Cheng; Ning Gu; Gaojun Teng; Qiujue Hu; Feipeng Zhu; Shuanghui Chang; Fan Zhang; Guangming Lu; Xiaoyuan Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging     Volume:  13     ISSN:  1860-2002     ISO Abbreviation:  Mol Imaging Biol     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-19     Completed Date:  2011-06-16     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101125610     Medline TA:  Mol Imaging Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-93     Citation Subset:  IM    
Radiology Department, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
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MeSH Terms
Brain Neoplasms / metabolism*,  pathology
Cell Line, Tumor
Ferritins / metabolism*
Ferumoxytol / metabolism*
Glioma / metabolism*,  pathology
Magnetic Resonance Imaging
Mice, Nude
Transplantation, Heterologous
Grant Support
Reg. No./Substance:
0/superparamagnetic blood pool agent; 1317-61-9/Ferumoxytol; 9007-73-2/Ferritins

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