Document Detail


Ferriprotoporphyrin IX binding substances and the mode of action of chloroquine against malaria.
MedLine Citation:
PMID:  6755119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine serum albumin and preparations of cell sap from malaria parasites and normal erythrocytes were tested for ability to protect cellular membranes against the toxicity of ferriprotoporphyrin IX (FP) and a chloroquine-FP complex. Suspensions of Plasmodium berghei (approximately 7 X 10(6) parasites per ml, isolated from saponin-lysed, infected erythrocytes) were used as a test system. Toxicity was monitored by measuring changes in turbidity of these suspensions at 700 nm. Parasite cell sap (0.56 mg protein per ml) and albumin (1 mg per ml) completely prevented the toxicity of 40 micrometers FP. Erythrocyte cell sap (8.6 mg of hemoglobin per ml). Provided only partial protection from 40 micrometers FP. Neither the cell sap preparations nor albumin eliminated the toxicity of a chloroquine-FP complex formed from 20 micrometers chloroquine and 40 micrometers FP. These observations suggest that the cell sap preparations contain FP binding substances and that the mode of action of chloroquine may be to shunt FP away from a nontoxic complex with these substances and into a toxic chloroquine-FP complex.
Authors:
H S Banyal; C D Fitch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  31     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1982 Sep 
Date Detail:
Created Date:  1983-01-19     Completed Date:  1983-01-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1141-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chloroquine / metabolism*,  therapeutic use*
Heme / analogs & derivatives*
Hemin / metabolism*,  toxicity
Malaria / drug therapy*,  parasitology
Plasmodium berghei / metabolism
Serum Albumin, Bovine / metabolism
Chemical
Reg. No./Substance:
0/Serum Albumin, Bovine; 14875-96-8/Heme; 16009-13-5/Hemin; 54-05-7/Chloroquine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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