Document Detail


Fermentations of pectin-rich biomass with recombinant bacteria to produce fuel ethanol.
MedLine Citation:
PMID:  10849785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pectin-rich residues from sugar beet processing contain significant carbohydrates and insignificant amounts of lignin. Beet pulp was evaluated for conversion to ethanol using recombinant bacteria as biocatalysts. Hydrolysis of pectin-rich residues followed by ethanolic fermentations by yeasts has not been productive because galacturonic acid and arabinose are not fermentable to ethanol by these organisms. The three recombinant bacteria evaluated in this study, Escherichia coli strain KO11, Klebsiella oxytoca strain P2, and Erwinia chrysanthemi EC 16 pLOI 555, ferment carbohydrates in beet pulp with varying efficiencies. E. coli KO11 is able to convert pure galacturonic acid to ethanol with minimal acetate production. Using an enzyme loading of 10.5 filter paper units of cellulase, 120.4 polygalacturonase units of pectinase, and 6.4 g of cellobiase (per gram of dry wt sugar beet pulp), with substrate addition after 24 h of fermentation, 40 g of ethanol/L was produced. Other recombinants exhibited lower ethanol yields with increases in acetate and succinate production.
Authors:
J B Doran; J Cripe; M Sutton; B Foster
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  84-86     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2000  
Date Detail:
Created Date:  2000-09-26     Completed Date:  2000-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  141-52     Citation Subset:  IM    
Affiliation:
Department of Biology, Central Michigan University, Mt. Pleasant 48859, USA. joy.doran@cmich.edu
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MeSH Terms
Descriptor/Qualifier:
Alcohol Dehydrogenase / genetics,  metabolism*
Biomass*
Escherichia coli / genetics,  physiology*
Ethanol*
Fermentation
Hexuronic Acids / metabolism
Kinetics
Klebsiella / genetics,  physiology*
Pectins*
Pectobacterium chrysanthemi / genetics,  physiology*
Pyruvate Decarboxylase / genetics,  metabolism*
Recombination, Genetic
Chemical
Reg. No./Substance:
0/Hexuronic Acids; 0/Pectins; 14982-50-4/galacturonic acid; 64-17-5/Ethanol; 9000-69-5/pectin; EC 1.1.1.1/Alcohol Dehydrogenase; EC 4.1.1.1/Pyruvate Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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