Document Detail

Fermentation of trans-aconitate via citrate, oxaloacetate, and pyruvate by Acidaminococcus fermentans.
MedLine Citation:
PMID:  8929281     Owner:  NLM     Status:  MEDLINE    
Growing cells of Acidaminococcus fermentans (DSM 20731 and ATCC 25085) fermented trans-aconitate via citrate, oxaloacetate, and pyruvate to approximately 2 CO2, 1.8 acetate, 0.1 butyrate and 0.9 H2. The carbon and electron recoveries were close to 100%. On citrate no growth was observed and washed cells were unable to ferment this tricarboxylate. In cell-free extracts, however, citrate as well as trans-aconitate were readily fermented to CO2 and acetate. Under these conditions, also cis-aconitate, oxaloacetate, and pyruvate were formed, whereas butyrate and intermediates of glutamate fermentation, 2-oxoglutatrate and glutaconate, could not be detected. Citrate Si-lyase, a Mg2+-dependent oxaloacetate decarboxylase, and pyruvate synthase were present in quantities that corresponded to the growth rate of the organism.
U Härtel; W Buckel
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of microbiology     Volume:  166     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-02-03     Completed Date:  1997-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  342-9     Citation Subset:  IM    
Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität, D-35032 Marburg, Germany.
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MeSH Terms
Aconitic Acid / metabolism*
Cell Extracts
Chromatography, Gas
Citric Acid / metabolism*
Glutamic Acid / metabolism
Gram-Negative Anaerobic Bacteria / metabolism*
Oxaloacetates / metabolism*
Oxaloacetic Acids
Pyruvic Acid / metabolism*
Reg. No./Substance:
0/Cell Extracts; 0/Oxaloacetates; 0/Oxaloacetic Acids; 127-17-3/Pyruvic Acid; 499-12-7/Aconitic Acid; 56-86-0/Glutamic Acid; 77-92-9/Citric Acid

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