Document Detail


Fermentation, hydrogen, and sulfur metabolism in multiple uncultivated bacterial phyla.
MedLine Citation:
PMID:  23019650     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BD1-5, OP11, and OD1 bacteria have been widely detected in anaerobic environments, but their metabolisms remain unclear owing to lack of cultivated representatives and minimal genomic sampling. We uncovered metabolic characteristics for members of these phyla, and a new lineage, PER, via cultivation-independent recovery of 49 partial to near-complete genomes from an acetate-amended aquifer. All organisms were nonrespiring anaerobes predicted to ferment. Three augment fermentation with archaeal-like hybrid type II/III ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) that couples adenosine monophosphate salvage with CO(2) fixation, a pathway not previously described in Bacteria. Members of OD1 reduce sulfur and may pump protons using archaeal-type hydrogenases. For six organisms, the UGA stop codon is translated as tryptophan. All bacteria studied here may play previously unrecognized roles in hydrogen production, sulfur cycling, and fermentation of refractory sedimentary carbon.
Authors:
Kelly C Wrighton; Brian C Thomas; Itai Sharon; Christopher S Miller; Cindy J Castelle; Nathan C VerBerkmoes; Michael J Wilkins; Robert L Hettich; Mary S Lipton; Kenneth H Williams; Philip E Long; Jillian F Banfield
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  337     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2012-10-02     Revised Date:  2012-11-20    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1661-5     Citation Subset:  IM    
Affiliation:
Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AMFJ00000000
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Archaeal Proteins / chemistry,  genetics,  metabolism
Bacteria, Anaerobic / classification*,  enzymology*,  genetics
Codon, Terminator / genetics
DNA, Bacterial
Fermentation
Genome, Bacterial
Hydrogen / metabolism*
Hydrogenase / chemistry,  genetics,  metabolism*
Molecular Sequence Data
Oxidation-Reduction
Phylogeny
Ribulose-Bisphosphate Carboxylase / chemistry,  genetics,  metabolism*
Sulfur / metabolism*
Tryptophan / genetics
Chemical
Reg. No./Substance:
0/Archaeal Proteins; 0/Codon, Terminator; 0/DNA, Bacterial; 1333-74-0/Hydrogen; 73-22-3/Tryptophan; 7704-34-9/Sulfur; EC 1.12.7.2/Hydrogenase; EC 4.1.1.39/Ribulose-Bisphosphate Carboxylase
Comments/Corrections
Erratum In:
Science. 2012 Nov 9;338(6108):742

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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