| Fermentation, hydrogen, and sulfur metabolism in multiple uncultivated bacterial phyla. | |
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MedLine Citation:
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PMID: 23019650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BD1-5, OP11, and OD1 bacteria have been widely detected in anaerobic environments, but their metabolisms remain unclear owing to lack of cultivated representatives and minimal genomic sampling. We uncovered metabolic characteristics for members of these phyla, and a new lineage, PER, via cultivation-independent recovery of 49 partial to near-complete genomes from an acetate-amended aquifer. All organisms were nonrespiring anaerobes predicted to ferment. Three augment fermentation with archaeal-like hybrid type II/III ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) that couples adenosine monophosphate salvage with CO(2) fixation, a pathway not previously described in Bacteria. Members of OD1 reduce sulfur and may pump protons using archaeal-type hydrogenases. For six organisms, the UGA stop codon is translated as tryptophan. All bacteria studied here may play previously unrecognized roles in hydrogen production, sulfur cycling, and fermentation of refractory sedimentary carbon. |
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Authors:
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Kelly C Wrighton; Brian C Thomas; Itai Sharon; Christopher S Miller; Cindy J Castelle; Nathan C VerBerkmoes; Michael J Wilkins; Robert L Hettich; Mary S Lipton; Kenneth H Williams; Philip E Long; Jillian F Banfield |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 337 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-09-28 Completed Date: 2012-10-02 Revised Date: 2012-11-20 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 1661-5 Citation Subset: IM |
Affiliation:
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Department of Earth and Planetary Science, University of California, Berkeley, Berkeley, CA 94720, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AMFJ00000000 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Archaeal Proteins / chemistry, genetics, metabolism Bacteria, Anaerobic / classification*, enzymology*, genetics Codon, Terminator / genetics DNA, Bacterial Fermentation Genome, Bacterial Hydrogen / metabolism* Hydrogenase / chemistry, genetics, metabolism* Molecular Sequence Data Oxidation-Reduction Phylogeny Ribulose-Bisphosphate Carboxylase / chemistry, genetics, metabolism* Sulfur / metabolism* Tryptophan / genetics |
| Chemical | |
Reg. No./Substance:
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0/Archaeal Proteins; 0/Codon, Terminator; 0/DNA, Bacterial; 1333-74-0/Hydrogen; 73-22-3/Tryptophan; 7704-34-9/Sulfur; EC 1.12.7.2/Hydrogenase; EC 4.1.1.39/Ribulose-Bisphosphate Carboxylase |
| Comments/Corrections | |
Erratum In:
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Science. 2012 Nov 9;338(6108):742 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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