Document Detail

Fermentability of grape must after inhibition with dimethyl dicarbonate (DMDC).
MedLine Citation:
PMID:  12236685     Owner:  NLM     Status:  MEDLINE    
Dimethyl dicarbonate (DMDC) was added to grape must and to synthetic media and results showed that, at 20 degrees C, 150 mg.L(-)(1) DMDC completely inhibited the fermentation of a grape must that was previously inoculated with 10(6) cells.mL(-)(1) Saccharomyces bayanus and Saccharomyces uvarum. Brettanomyces intermedius, Candida guilliermondii, Hansenula jadinii, Hansenula petersonii, Kloeckera apiculata, Pichia membranaefaciens, and Saccharomyces cerevisiae were inhibited by 250 mg.L(-)(1). Candida valida was inhibited in the presence of 350 mg.L(-)(1), whereas Hanseniaspora osmophila, Saccharomycodes ludwigii, Schizosaccharomyces pombe, and Zygosaccharomyces bailii required 400 mg.L(-)(1). Delay of fermentation (but not inhibition) was noted in the presence of 400 mg.L(-)(1) for the following cultures: Brettanomyces anomalus, Hanseniaspora uvarum, Metschnikowia pulcherrima, Schizosaccharomyces japonicus, Torulaspora delbrueckii, and Zygosaccharomyces florentinus. Acetobacter aceti and Lactobacillus sp. were completely inhibited using 1000 and 500 mg.L(-)(1) DMDC, respectively. The fermentation of a grape must inoculated with 10(6) cells.mL(-)(1) of different wine yeasts was delayed for 4 days after the prior addition of 200 mg.L(-)(1) of DMDC; 200 mg.L(-)(1) DMDC did not show any residual inhibitory effect after 12 h, nor did 300 mg.L(-)(1) 24 h after the addition. In cellar experiments, indigenously contaminated grape musts (with and without skins) showed a delay in fermentation of 48 h after the addition of only 50 mg.L(-)(1) DMDC. The possibility of using DMDC (as pure grade as commercially available) in grape must as a disinfectant for the decontamination of musts indigenously contaminated with wild yeast should be considered seriously, despite its apparent low solubility in water.
Claudio Delfini; Piero Gaia; Raffaella Schellino; Morela Strano; Adolfo Pagliara; Stefano Ambrò
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  50     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-18     Completed Date:  2002-11-05     Revised Date:  2013-01-28    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5605-11     Citation Subset:  IM    
Istituto Sperimentale per l'Enologia, Sezione di Microbiologia, via P. Micca 35, 14100 Asti, Italy.
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MeSH Terms
Acetobacter / metabolism
Candida / metabolism
Diethyl Pyrocarbonate / analogs & derivatives*,  pharmacology*
Food Handling
Lactobacillus / metabolism
Pichia / metabolism
Saccharomyces / metabolism
Saccharomyces cerevisiae / metabolism
Vitis / metabolism*,  microbiology*
Wine / microbiology*
Yeasts / metabolism
Reg. No./Substance:
1609-47-8/Diethyl Pyrocarbonate; 1AY9229ZMG/dimethyl pyrocarbonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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