| Femtosecond protein nanocrystallography-data analysis methods. | |
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MedLine Citation:
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PMID: 20389587 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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X-ray diffraction patterns may be obtained from individual submicron protein nanocrystals using a femtosecond pulse from a free-electron X-ray laser. Many "single-shot" patterns are read out every second from a stream of nanocrystals lying in random orientations. The short pulse terminates before significant atomic (or electronic) motion commences, minimizing radiation damage. Simulated patterns for Photosystem I nanocrystals are used to develop a method for recovering structure factors from tens of thousands of snapshot patterns from nanocrystals varying in size, shape and orientation. We determine the number of shots needed for a required accuracy in structure factor measurement and resolution, and investigate the convergence of our Monte-Carlo integration method. |
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Authors:
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Richard A Kirian; Xiaoyu Wang; Uwe Weierstall; Kevin E Schmidt; John C H Spence; Mark Hunter; Petra Fromme; Thomas White; Henry N Chapman; James Holton |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Optics express Volume: 18 ISSN: 1094-4087 ISO Abbreviation: Opt Express Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-07-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101137103 Medline TA: Opt Express Country: United States |
Other Details:
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Languages: eng Pagination: 5713-23 Citation Subset: IM |
Affiliation:
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Department of Physics, Arizona State University, Tempe, Arizona 85287 USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Algorithms* Pattern Recognition, Automated / methods* Photosystem I Protein Complex / ultrastructure* X-Ray Diffraction / methods* |
| Chemical | |
Reg. No./Substance:
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0/Photosystem I Protein Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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