| Femtosecond optical transfection of cells: viability and efficiency. | |
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MedLine Citation:
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PMID: 19529083 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 microJ/cm(2), have found an average transfection efficiency of 50 +/- 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique. |
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Authors:
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D Stevenson; B Agate; X Tsampoula; P Fischer; C T A Brown; W Sibbett; A Riches; F Gunn-Moore; K Dholakia |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Optics express Volume: 14 ISSN: 1094-4087 ISO Abbreviation: Opt Express Publication Date: 2006 Aug |
Date Detail:
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Created Date: 2009-06-16 Completed Date: 2009-09-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101137103 Medline TA: Opt Express Country: United States |
Other Details:
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Languages: eng Pagination: 7125-33 Citation Subset: IM |
Affiliation:
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Interdisciplinary Centre for Medical Photonics, School of Physics & Astronomy, University of St Andrews, St Andrews, KY16 9SS, Scotland, UK. ds50@st-andrews.ac.uk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival / physiology* Cricetinae Female Lasers, Semiconductor Optical Phenomena Optical Tweezers Ovary / cytology* Transfection / methods* |
| Grant Support | |
ID/Acronym/Agency:
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//Medical Research Council |
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