Document Detail


Femtosecond optical transfection of cells: viability and efficiency.
MedLine Citation:
PMID:  19529083     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 microJ/cm(2), have found an average transfection efficiency of 50 +/- 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique.
Authors:
D Stevenson; B Agate; X Tsampoula; P Fischer; C T A Brown; W Sibbett; A Riches; F Gunn-Moore; K Dholakia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Optics express     Volume:  14     ISSN:  1094-4087     ISO Abbreviation:  Opt Express     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2009-06-16     Completed Date:  2009-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137103     Medline TA:  Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7125-33     Citation Subset:  IM    
Affiliation:
Interdisciplinary Centre for Medical Photonics, School of Physics & Astronomy, University of St Andrews, St Andrews, KY16 9SS, Scotland, UK. ds50@st-andrews.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Survival / physiology*
Cricetinae
Female
Lasers, Semiconductor
Optical Phenomena
Optical Tweezers
Ovary / cytology*
Transfection / methods*
Grant Support
ID/Acronym/Agency:
//Medical Research Council

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