Document Detail

Femtosecond laser-assisted microinjection into living neurons.
MedLine Citation:
PMID:  18687359     Owner:  NLM     Status:  MEDLINE    
A Ti:sapphire femtosecond laser micro-manipulation system was used to perforate vital cells. Propidium iodide was successfully transferred into both astrocytes and PC12 cells via the channel of a femtosecond laser, which induced transiently recoverable opening of the cell membrane. The transfection efficiency reached almost 100%. This method also had the advantage of contact-free, non-disruptive and stable transfection.
Ming Lei; Hanpeng Xu; Hao Yang; Baoli Yao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-18
Journal Detail:
Title:  Journal of neuroscience methods     Volume:  174     ISSN:  0165-0270     ISO Abbreviation:  J. Neurosci. Methods     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-12     Completed Date:  2008-11-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905558     Medline TA:  J Neurosci Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  215-8     Citation Subset:  IM    
State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
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MeSH Terms
Aluminum Oxide
Microinjections / instrumentation*,  methods*
Microscopy, Confocal
Neurons / physiology*
PC12 Cells
Reg. No./Substance:
1344-28-1/Aluminum Oxide; 7440-32-6/Titanium

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