Document Detail


Female parthenogenetic apomixis and androsporogenetic parthenogenesis in embryonal cells of Araucaria angustifolia: interpolation of progenesis and asexual heterospory in an artificial sporangium.
MedLine Citation:
PMID:  22669467     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cell fate, development timing and occurrence of reproductive versus apomictic development in gymnosperms are shown to be influenced by culture conditions in vitro. In this study, female parthenogenetic apomixis (fPA), androsporogenetic parthenogenesis (mAP) and progenesis were demonstrated using embryonal initials of Araucaria angustifolia in scaled-up cell suspensions passing through a single-cell bottleneck in darkness and in an artificial sporangium (AS). Expression was based on defined nutrition, hormones and feedforward-adaptive feedback process controls at 23-25 °C and in darkness. In fPA, the nucleus of an embryonal initial undergoes endomitosis and amitosis, forming a diploid egg-equivalent and an apoptotic ventral canal nucleus in a transdifferentiated archegonial tube. Discharge of egg-equivalent cells as parthenospores and their dispersal into the aqueous culture medium were followed by free-nuclear conifer-type proembryogenesis. This replaced the plesiomorphic and central features of proembryogenesis in Araucariaceae. Protoplasmic fusions of embryonal initials were used to reconstruct heterokaryotic expressions of fPA in multiwell plates. In mAP, restitutional meiosis (automixis) was responsible for androsporogenesis and the discharge of monads, dyads, tetrads and polyads. In a display of progenesis, reproductive development was brought to an earlier ontogenetic stage and expressed by embryonal initials. Colchicine increased polyploidy, but androspore formation became aberrant and fragmented. Aberrant automixis led to the formation of chromosomal bouquets, which contributed to genomic silencing in embryonal initials, cytomixis and the formation of pycnotic micronucleated cells. Dispersal of female and male parthenospores displayed heteromorphic asexual heterospory in an aqueous environment.
Authors:
Don J Durzan
Related Documents :
22610977 - Synergistic effects of amyloid peptides and lead on human neuroblastoma cells.
22552967 - Mmneu3 sialidase over-expression in c2c12 myoblasts delays differentiation and induces ...
6573827 - Cytologic evaluation of atypical cells in cervicovaginal smears from women with tubal p...
1622117 - Fluorescent location of abnormal cells in cell smears obtained from the lungs of patien...
17190787 - Characteristic expression of lewis-antigenic glycolipids in human ovarian carcinoma-der...
9611127 - Glutamate transport and cellular glutamine metabolism: regulation in llc-pk1 vs. llc-pk...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-5
Journal Detail:
Title:  Sexual plant reproduction     Volume:  -     ISSN:  1432-2145     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955730     Medline TA:  Sex Plant Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
University of California, Plant Sciences, MS 6, Old Davis Rd, Davis, CA, 95616, USA, djdurzan@ucdavis.edu.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The B-cell identity factor Pax5 regulates distinct transcriptional programmes in early and late B ly...
Next Document:  Undercarboxylated osteocalcin is positively associated with free testosterone in male patients with ...