Document Detail


Female sex bias in human embryonic stem cell lines.
MedLine Citation:
PMID:  21585244     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The factors limiting the rather inefficient derivation of human embryonic stem cells (HESCs) are not fully understood. The aim of this study was to analyze the sex ratio in our 42 preimplantation genetic diagnosis (PGD)-HESC lines, in an attempt to verify its affect on the establishment of HESC lines. The ratio between male and female PGD-derived cell lines was compared. We found a significant increase in female cell lines (76%). This finding was further confirmed by a meta-analysis for combining the results of all PGD-derived HESC lines published to date (148) and all normal karyotyped HESC lines derived from spare in vitro fertilization embryos worldwide (397). Further, gender determination of embryos demonstrated that this difference originates from the actual derivation process rather than from unequal representation of male and female embryos. It can therefore be concluded that the clear-cut tendency for female preponderance is attributed to suboptimal culture conditions rather than from a true gender imbalance in embryos used for derivation of HESC lines. We propose a mechanism in which aberrant X chromosome inactivation and/or overexpression of critical metabolic X-linked genes might explain this sex dimorphism.
Authors:
Dalit Ben-Yosef; Ami Amit; Mira Malcov; Tsvia Frumkin; Ahmi Ben-Yehudah; Ido Eldar; Nava Mey-Raz; Foad Azem; Gheona Altarescu; Paul Renbaum; Rachel Beeri; Irit Varshaver; Talia Eldar-Geva; Silvina Epsztejn-Litman; Ephrat Levy-Lahad; Rachel Eiges
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Publication Detail:
Type:  Comparative Study; Journal Article; Meta-Analysis; Research Support, Non-U.S. Gov't     Date:  2011-06-24
Journal Detail:
Title:  Stem cells and development     Volume:  21     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-01     Completed Date:  2012-05-22     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  363-72     Citation Subset:  IM    
Affiliation:
Department of Cell and Developmental Biology Sackler Medical School, Tel Aviv Sourasky Medical Center, Tel Aviv University, Lis Maternity Hospital, Tel Aviv, Israel.
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / analysis
Cell Line
Chromosomes, Human / physiology
Embryo, Mammalian / cytology,  physiology*
Embryonic Development
Embryonic Stem Cells / cytology*,  physiology
Female
Genome, Human
Humans
Karyotype
Male
Preimplantation Diagnosis
Sex Ratio*
X Chromosome Inactivation
Chemical
Reg. No./Substance:
0/Biological Markers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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