| Feeding rats a high fat/carbohydrate ratio diet reduces jejunal S/I activity ratio and unsialylated galactose on glycosylated chain of S-I complex. | |
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MedLine Citation:
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PMID: 20159024 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: We examined whether decreasing jejunal sucrase/isomaltase (S/I) activity ratio by feeding rats a high fat/carbohydrate ratio diet is regulated by changing glycosylated chains on the S-I complex. MAIN METHODS: Jejunal activities of sucrase, isomaltase and beta-1,4-galactosyltransferase were examined in rats fed a high fat/carbohydrate or a low fat/carbohydrate ratio diet. The amount of galactose and mannose in the glycosylated chain on the S-I complex in rats fed both diets was determined using RCA(120) and Con A lectins, respectively. The effects of reducing unsialylated galactose from the glycosylated chain on the S-I complex were assessed by determining sucrase activity in purified S-I complex treated with beta-galactosidase. KEY FINDINGS AND SIGNIFICANCE: Feeding rats a high fat/carbohydrate ratio diet reduced jejunal S/I activity ratio in mucosal homogenates and purified fractions. The level of unsialylated galactose in glycosylated chains on the S-I complex was reduced by feeding rats a high fat/carbohydrate ratio diet. The form with reduced levels of unsialylated galactose had lower sucrase activity than that with more unsialylated galactose. The reduction of galactose on the S-I complex by beta-galactosidase in vitro reduced sucrase activity. Feeding rats a high fat/carbohydrate ratio diet also reduced jejunal beta-1,4-galactosyltransferase activity. Taken together, decreasing the S/I activity ratio by feeding rats a high fat/carbohydrate diet is associated with the reduction of unsialylated galactose on the glycosylated chain of the S-I complex. |
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Authors:
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Kazuki Mochizuki; Makiko Igawa-Tada; Sachiko Takase; Toshinao Goda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-14 |
Journal Detail:
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Title: Life sciences Volume: 86 ISSN: 1879-0631 ISO Abbreviation: Life Sci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-22 Completed Date: 2010-04-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 524-31 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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The University of Shizuoka, Graduate School of Nutritional and Environmental Sciences and Global COE, Shizuoka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Diet* Dietary Carbohydrates / pharmacology* Dietary Fats / pharmacology* Electrophoresis, Polyacrylamide Gel Galactose / metabolism* Glycosylation Jejunum / drug effects* Male N-Acetyllactosamine Synthase / genetics, metabolism RNA, Messenger / genetics, metabolism Rats Rats, Sprague-Dawley Sucrase-Isomaltase Complex / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Dietary Carbohydrates; 0/Dietary Fats; 0/RNA, Messenger; 26566-61-0/Galactose; EC 2.4.1.90/N-Acetyllactosamine Synthase; EC 3.2.1.-/Sucrase-Isomaltase Complex |
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