| Feeding entrainment of food-anticipatory activity and per1 expression in the brain and liver of zebrafish under different lighting and feeding conditions. | |
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MedLine Citation:
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PMID: 20795882 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Food provided on a periodic basis can act as a potent synchronizer, being a stronger zeitgeber than light for peripheral oscillators in mammals. In fish, however, little is known about the influence of feeding time on the circadian pacemaker and the relationship between central and peripheral oscillators. The aim of this research was to investigate the influence of mealtime on the activity rhythms, and on central (brain) and peripheral (liver) oscillators in zebrafish. The authors tested different feeding times under a light-dark (LD) cycle and the endogenous origin of food-anticipatory activity (FAA) by feeding zebrafish at a fixed time under constant bright-light conditions (LL). The authors then measured locomotor activity and the expression of the clock gene per1 in animals under a LD cycle and fed at random times during the light phase, with restricted feeding at the mid-light phase (ML) or with restricted feeding during the mid-dark phase (MD). Finally, the authors measured locomotor activity and per1 expression in fish maintained under LL under either random feeding or scheduled feeding. Zebrafish displayed FAA in all the groups fed at a fixed time but not when feeding was randomly scheduled. Under LL, fish entrainment persisted, and when released under fasting conditions FAA free-ran with a circa-24-h period. The expression of per1 in the brain of fish under LD showed a daily rhythm with the acrophase (peak time) at the end of the dark phase regardless of feeding schedule. This brain rhythm disappeared in LL fish under both random feeding and scheduled feeding. Feeding at MD advanced the phase of per1 in the liver by 7 h compared with the ML-fed group phase (23:54 versus 07:23 h, respectively). In addition, under LL scheduled feeding entrained the rhythms of per1 expression in the liver. This study reveals for the first time that scheduled feeding entrains peripheral oscillators in a fish species, zebrafish, which is a powerful model widely used for molecular genetics and for the study of basic clock mechanisms of the vertebrate circadian system. |
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Authors:
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Jose F López-Olmeda; Erica V Tartaglione; Horacio O de la Iglesia; Francisco J Sánchez-Vázquez |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chronobiology international Volume: 27 ISSN: 1525-6073 ISO Abbreviation: Chronobiol. Int. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-27 Completed Date: 2010-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8501362 Medline TA: Chronobiol Int Country: England |
Other Details:
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Languages: eng Pagination: 1380-400 Citation Subset: IM |
Affiliation:
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Department of Physiology, Faculty of Biology, University of Murcia, Murcia, Spain. jflopez@um.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticipation, Psychological Brain / metabolism* Circadian Rhythm / physiology Feeding Behavior / physiology* Light* Lighting Liver / metabolism* Motor Activity / physiology Period Circadian Proteins / genetics, metabolism* Zebrafish / anatomy & histology, physiology* Zebrafish Proteins / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Period Circadian Proteins; 0/Zebrafish Proteins; 0/per1a protein, zebrafish |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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