Document Detail

Feedback regulation of SREBP and aromatase in A beta(25-35)-supplemented human neuroblastoma cells.
MedLine Citation:
PMID:  16767510     Owner:  NLM     Status:  MEDLINE    
1. The analogies between the processing of amyloid precursor protein (APP) and other transmembrane sterol regulatory element binding proteins (SREBPs) inspired us to conduct further studies on whether beta-amyloid (Abeta) affects aromatase by interacting with APP and SREBP. 2. In this study, cultured human neuroblastoma cells (SHSY-5Y) were incubated in experimental media (media without FBS, the main cholesterol source) in the presence or absence of Abeta (1 microM) for 24 h. 3. Cellular extracts were subjected to immunoblot analysis using anti-APP, anti-aromatase and anti-SREBP-1. In these cell lines, we detected aromatase (55 kDa), SREBP cleavage product (68 kDa) and APP precursor (100-95 kDa) and cleavage product (60 kDa) by immunoblotting. Aromatase and SREBP levels were elevated in the cells incubated 24 h in experimental media and were attenuated in Abeta-supplemented experimental media. 4. The disturbance of cholesterol homeostasis appears to be an important factor in the pathogenesis of Alzheimer's disease. These findings may have important implications for understanding the mechanisms of the aromatase enzyme gene in disease states such as Alzheimer's.
Pelin Kelicen; Agneta Nordberg
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-06
Journal Detail:
Title:  Cellular and molecular neurobiology     Volume:  26     ISSN:  0272-4340     ISO Abbreviation:  Cell. Mol. Neurobiol.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-06-12     Completed Date:  2007-07-02     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8200709     Medline TA:  Cell Mol Neurobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  225-35     Citation Subset:  IM    
Faculty of Pharmacy, Department of Pharmacology, Hacettepe University, 06100 Sihhiye, Ankara, Turkey.
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MeSH Terms
Amyloid beta-Protein / metabolism*
Amyloid beta-Protein Precursor / metabolism
Aromatase / chemistry,  metabolism*
Cell Line, Tumor
Culture Media / chemistry
Feedback, Physiological*
Molecular Weight
Neuroblastoma / metabolism*
Peptide Fragments / metabolism*
Sterol Regulatory Element Binding Proteins / chemistry,  metabolism*
Reg. No./Substance:
0/Amyloid beta-Protein; 0/Amyloid beta-Protein Precursor; 0/Culture Media; 0/Peptide Fragments; 0/Sterol Regulatory Element Binding Proteins; 0/amyloid beta-protein (25-35); EC

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