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Feed efficiency: mitochondrial function to global gene expression.
MedLine Citation:
PMID:  23148240     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Understanding the cellular basis of feed efficiency (FE) is instrumental to helping poultry and livestock industries continue to provide high quality protein for an increasingly crowded world. To understand relationships of FE and gene expression, global RNA transcription was investigated in breast muscle obtained from a male broiler line fed the same diet and individually phenotyped for FE. In these studies, RNA samples obtained from broilers that exhibited either high FE (0.65 ± 0.01) or low FE (0.46 ± 0.01) were analyzed with an Agilent 44K chicken oligoarray. A 1.3-fold cutoff in expression (30% difference between groups) resulted in 782 genes that were differentially expressed (P < 0.05) in muscle between the high and low FE phenotypes. Ingenuity Pathway Analysis, an online software program, was used to identify, genes, gene networks, and pathways associated with the phenotypic expression of FE. The results indicate that the high FE phenotype exhibited increased expression of genes associated with:1) signal transduction pathways; 2) anabolic activities; and 3) energy sensing and energy coordination activities, all of which would likely be favorable to cell growth and development. In contrast, the low FE broiler phenotype exhibited up-regulation of genes: 1) associated with actin-myosin filaments; cytoskeletal architecture, and(or) muscle fibers, and 2) stress-related or stress-responsive genes. Since the low FE broiler phenotype exhibits greater oxidative stress, it would appear that low FE phenotype is the product of inherent gene expression that is modulated by oxidative stress. The results of these studies begin to provide a comprehensive picture of gene expression in muscle, a major organ of energy demand in an animal, associated with phenotypic expression of FE.
Authors:
W Bottje; B-W Kong
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-12
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Poultry Science, Center of Excellence for Poultry Science, Division of Agriculture, University of Arkansas, Fayetteville 72701.
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