Document Detail

Fed-batch production of citric acid by Candida lipolytica grown on n-paraffins.
MedLine Citation:
PMID:  15099907     Owner:  NLM     Status:  MEDLINE    
This study reports on the effects of fermentor agitation and fed-batch mode of operation on citric acid production from Candida lipolytica using n-paraffin as the carbon source. An optimum range of agitation speeds in the 800-1000 rpm range corresponding to Reynolds numbers of 50000-63000 (based on initial batch conditions) seemed to give the best balance between substrate utilization for biomass growth and citric acid production. Application of multiple fed-batch feedings can be used to extend the batch fermentation and increase final citric acid concentrations and product yield. The three-cycle fed-batch system increased overall citric acid yields to 0.8-1.0 g citricacid/g n-paraffin, approximately a 100% improvement in product yield from those observed in the single cycle fed-batch system and a 200% improvement over normal batch operation. The three-cycle fed-batch mode of operation also increased the final citric acid concentration to 42 g/l from about 12 and 6g/l for single fed-batch cycle and normal batch modes of operation, respectively. Increased citric acid concentrations in three-cycle fed-batch mode was achieved at longer fermentation times.
A Crolla; K J Kennedy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biotechnology     Volume:  110     ISSN:  0168-1656     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-04-21     Completed Date:  2005-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  73-84     Citation Subset:  IM    
Department of Chemical Engineering, University of Ottawa, Ottawa, Ont., Canada K1N 6N5.
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MeSH Terms
Candida / growth & development,  metabolism*
Cell Culture Techniques
Citric Acid / isolation & purification,  metabolism*
Paraffin / metabolism*,  pharmacology
Time Factors
Reg. No./Substance:
77-92-9/Citric Acid; 8002-74-2/Paraffin

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