| Fed-batch production of a bioflocculant from Corynebacterium glutamicum. | |
| | |
MedLine Citation:
|
PMID: 20589412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The constant-rate fed-batch production of the polygalacturonic acid bioflocculant REA-11 was studied. A controlled sucrose-feeding strategy resulted in a slight improvement in biomass and a 7% reduction in flocculating activity compared with the batch process. When fed with a 3 g l(-1) urea solution, the flocculating activity was enhanced to 720 U ml(-1) in 36 h. High cell density (2.12 g l(-1)) and flocculating activity (820 U ml(-1)) were obtained in a 10-l fermentor by feeding with a sucrose-urea solution, with values of nearly two times and 50% higher than those of the batch process, respectively. Moreover, the residual sucrose declined to 2.4 g l(-1), and residual urea decreased to 0.03 g l(-1). Even higher flocculating activity of 920 U ml(-1) and biomass of 3.26 g l(-1) were obtained by feeding with a sucrose-urea solution in a pilot scale fermentation process, indicating the potential industrial utility of this constant-rate feeding strategy in bioflocculant production by Corynebacterium glutamicum. |
| | |
Authors:
|
Haijun Wu; Qingbiao Li; Rui Lu; Yuanpeng Wang; Xiaoling Zhuang; Ning He |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-30 |
Journal Detail:
|
Title: Journal of industrial microbiology & biotechnology Volume: 37 ISSN: 1476-5535 ISO Abbreviation: J. Ind. Microbiol. Biotechnol. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-10-18 Completed Date: 2011-01-27 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: England |
Other Details:
|
Languages: eng Pagination: 1203-9 Citation Subset: IM |
Affiliation:
|
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Biomass* Bioreactors Corynebacterium glutamicum / metabolism* Culture Media, Conditioned / chemistry Fermentation* Flocculation Genetic Engineering Glucose / metabolism Pectins / biosynthesis* Sucrose / metabolism Urea / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media, Conditioned; 0/Pectins; 50-99-7/Glucose; 57-13-6/Urea; 57-50-1/Sucrose; 9046-38-2/polygalacturonic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The determinants of sexually transmitted infections among reproductive age women in St. Petersburg, ...
Next Document: The effect of divalent cations on the catalytic activity of the human plasma 3'-exonuclease.