Document Detail


Fecal water genotoxicity is predictive of tumor-preventive activities by inulin-like oligofructoses, probiotics (Lactobacillus rhamnosus and Bifidobacterium lactis), and their synbiotic combination.
MedLine Citation:
PMID:  15489207     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The measurement of fecal water genotoxicity in human colon cells could be a useful biomarker to study effects of diet in the colon. Here we assessed aqueous fecal extracts of samples from a chronic study with rats fed prebiotics, probiotics, and their combination. Treatments were maltodextrins (controls), inulin/oligofructoses (prebiotic), Lactobacillus rhamnosus, and Bifidobacterium lactis (probiotics) or both (synbiotic). Azoxymethane (AOM) was administered to initiate tumors. Rat feces were collected at 0 and 10 days and 2, 4, and 8 mo, and cecal contents were collected at 8 mo. Aqueous phases were prepared and tested for genotoxicity in HT29 colon cells using the comet assay. The studied types of intervention reduced fecal and cecal genotoxicity. DNA damage by samples from AOM-treated, tumor-free rats was significantly lower than from tumor-bearing animals, especially after 4 mo of synbiotic and prebiotic interventions. Inulin-based diets reduced exposure to genotoxins in the feces, directly reflecting the reported reduction of tumor incidence in these animals. Evidence is provided for the validity of this measurement as a biomarker of chemoprevention because 1) fecal water genotoxicity reflected genotoxic exposure in the cecum, 2) tumor incidence and fecal genotoxicity were directly related, and 3) these interventions reduced tumor risks by reducing exposure to genotoxins in the gut.
Authors:
Annett Klinder; Antje Förster; Giovanna Caderni; Angelo Pietro Femia; Beatrice L Pool-Zobel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrition and cancer     Volume:  49     ISSN:  0163-5581     ISO Abbreviation:  Nutr Cancer     Publication Date:  2004  
Date Detail:
Created Date:  2004-10-18     Completed Date:  2005-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  144-55     Citation Subset:  IM    
Affiliation:
Department of Nutritional Toxicology, Institute for Nutrition, Friedrich Schiller University, Jena, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Azoxymethane / toxicity
Bifidobacterium / physiology
Biological Markers / analysis
Carcinogens / toxicity
Colonic Neoplasms / chemically induced,  epidemiology,  prevention & control*
Comet Assay
Feces / chemistry*
Gastrointestinal Contents
HT29 Cells
Humans
Lactobacillus / physiology
Male
Mutagenicity Tests
Oligosaccharides / pharmacology*
Probiotics / pharmacology*
Random Allocation
Rats
Rats, Inbred F344
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Carcinogens; 0/Oligosaccharides; 0/oligofructose; 25843-45-2/Azoxymethane

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