Document Detail

Fecal fat determination with a modified titration method.
MedLine Citation:
PMID:  20201717     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Intestinal malabsorption is a serious condition which unfortunately may remain unrecognized due to methodological problems. The old titration method of van de Kamer is still the gold standard. We present a modification of the method, pointing out practical improvements and pitfalls. MATERIAL AND METHODS: Our method uses less than one tenth of the amount of feces originally described, which implies proper mixing and homogenization of all feces collected over 72 hrs. Validation is performed by measuring fat concentration in commercial milk products. RESULTS: Reproducibility and validity were satisfactory. Concentration and output of fecal fat was poorly correlated, indicating that fecal output is required for diagnosing intestinal malabsorption. Our experiments also highlight the importance of using a non-polar extraction agent because polar agents take up water-soluble short chain fatty acids which are derived from fermentation of carbohydrates and give erroneous results. CONCLUSION: The modified method is reliable and robust and minimizes the aesthetical problems associated with fecal fat determination.
Arnold Berstad; Friedemann Erchinger; Aud-Sissel Hjartholm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  45     ISSN:  1502-7708     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-22     Completed Date:  2010-08-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  England    
Other Details:
Languages:  eng     Pagination:  603-7     Citation Subset:  IM    
Institute of Medicine, University of Bergen, Haukeland University Hospital, Bergen, Norway.
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MeSH Terms
Acetic Acid
Ether, Ethyl
Fats / analysis*
Feces / chemistry*
Malabsorption Syndromes / diagnosis*
Reproducibility of Results
Specimen Handling
Titrimetry / methods*
Reg. No./Substance:
0/Alkanes; 0/Fats; 0/Solvents; 60-29-7/Ether, Ethyl; 64-19-7/Acetic Acid; 8030-30-6/naphtha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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