| Fecal fat determination with a modified titration method. | |
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MedLine Citation:
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PMID: 20201717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Intestinal malabsorption is a serious condition which unfortunately may remain unrecognized due to methodological problems. The old titration method of van de Kamer is still the gold standard. We present a modification of the method, pointing out practical improvements and pitfalls. MATERIAL AND METHODS: Our method uses less than one tenth of the amount of feces originally described, which implies proper mixing and homogenization of all feces collected over 72 hrs. Validation is performed by measuring fat concentration in commercial milk products. RESULTS: Reproducibility and validity were satisfactory. Concentration and output of fecal fat was poorly correlated, indicating that fecal output is required for diagnosing intestinal malabsorption. Our experiments also highlight the importance of using a non-polar extraction agent because polar agents take up water-soluble short chain fatty acids which are derived from fermentation of carbohydrates and give erroneous results. CONCLUSION: The modified method is reliable and robust and minimizes the aesthetical problems associated with fecal fat determination. |
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Authors:
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Arnold Berstad; Friedemann Erchinger; Aud-Sissel Hjartholm |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Scandinavian journal of gastroenterology Volume: 45 ISSN: 1502-7708 ISO Abbreviation: Scand. J. Gastroenterol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-22 Completed Date: 2010-08-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060105 Medline TA: Scand J Gastroenterol Country: England |
Other Details:
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Languages: eng Pagination: 603-7 Citation Subset: IM |
Affiliation:
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Institute of Medicine, University of Bergen, Haukeland University Hospital, Bergen, Norway. arnold.berstad@med.uib.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid Alkanes Ether, Ethyl Fats / analysis* Feces / chemistry* Humans Malabsorption Syndromes / diagnosis* Reproducibility of Results Solvents Specimen Handling Titrimetry / methods* |
| Chemical | |
Reg. No./Substance:
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0/Alkanes; 0/Fats; 0/Solvents; 60-29-7/Ether, Ethyl; 64-19-7/Acetic Acid; 8030-30-6/naphtha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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