| Febrile response induced by cecal ligation and puncture (CLP) in rats: involvement of prostaglandin E(2) and cytokines. | |
| | |
MedLine Citation:
|
PMID: 22203392 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The purpose of the present study was to better understand the events involved in the febrile response induced by cecal ligation and puncture (CLP), a complex infectious process. To this end, we conducted in vivo experiments in rats examining (1) fever development, (2) bacterial number in the infection focus and in blood, (3) peripheral and hypothalamic synthesis of cytokines, (4) hypothalamic and cerebrospinal fluid (CSF) synthesis of prostaglandin E(2) (PGE(2)), (5) the effect of anti-IL-6 antibody on fever, and (6) the effect of celecoxib on fever and hypothalamic synthesis of PGE(2) after CLP induction. We found that CLP promotes fever and animal death depending on the number of punctures. The peak of CLP-induced fever overlapped with the maximal increase in the number of bacteria in the infectious focus and blood, which occurred at 6 and 12 h. The peak of the febrile response also coincided with increased amounts of interleukin (IL)-1β, IL-6 and IL-10 in the peritoneal exudate and serum; IL-6 in the hypothalamus and PGE(2) in the CSF and predominantly in the hypothalamus. Moreover, intracerebroventricularly injected anti-IL-6 antibody reduced the febrile response while celecoxib reduced the fever and PGE(2) amount in the hypothalamus induced by CLP. Tumor necrosis factor (TNF)-α peaked at 3 h at all sites studied. Conversely, IL-10 concentration decreased in the hypothalamus. These findings show that the peak of CLP-induced fever is accompanied by an increase of bacteria in peritoneal fluid (local infection) and blood; local synthesis of pyrogenic (IL-1β, IL-6) and antipyretic (IL-10) cytokines and central production of IL-6 and PGE(2), suggesting that these last are the central mediators of this response. |
| | |
Authors:
|
Maria José Figueiredo; Denis de Melo Soares; Juliano Manvailer Martins; Renes de Resende Machado; Carlos Arterio Sorgi; Lucia Helena Faccioli; Miriam Cristina Contin de Melo; David do Carmo Malvar; Glória E P Souza |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-28 |
Journal Detail:
|
Title: Medical microbiology and immunology Volume: - ISSN: 1432-1831 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2011-12-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0314524 Medline TA: Med Microbiol Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Laboratory of Pharmacology, Department of Physic and Chemistry, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. do Café, s/n, Campus Universitário, 14049-903, Ribeirão Preto, São Paulo, Brazil, zzfigueiredo@usp.br. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Risk factors that increase mortality after living donor liver transplantation.
Next Document: Effects of Different Substrate Composition on Biosynthesis of Polyhydroxybutyrate-co-hydroxyvalerate...