| Features of senescence and cell death induced by doxorubicin in A549 cells: organization and level of selected cytoskeletal proteins. | |
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MedLine Citation:
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PMID: 19898866 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Senescence and cell death are fail-safe mechanisms protecting against tumorigenesis. Both these forms of cellular response could be induced in cancer cells, thus suppressing tumor progression. Therefore, to fully understand chemotherapeutic effects, not only symptoms of cell death, but also of senescence should be evaluated. Since the involvement of cytoskeleton components in these processes has been reported, changes in the organization and level of some cytoskeletal proteins may be indicative of cell fate. METHODS: We analyzed selected markers of senescence and cell death, including possible alterations in vimentin and G-actin cytoskeleton in A549 cells after treatment with doxorubicin. Light (SA-beta-galactosidase), fluorescent (vimentin and G-actin labeling) and electron microscopic examinations along with flow cytometry methods (TUNEL, Annexin V/PI staining, cell cycle analysis, intracellular level of vimentin) were employed to determine the outcome of the treatment. RESULTS: Uncoupling between senescent cell morphology and stable cell cycle arrest occurred. Some differences in the organization and level of cytoskeletal proteins, especially of vimentin, like fluctuations in its level, were observed. On the other hand, G-actin seemed to be more stable than vimentin. CONCLUSIONS: G-actin stability may imply its potential usefulness for permanent senescence detection. Along with slight to moderate cytoskeletal alterations, the obtained results suggest transient senescence-like state induction, followed by morphology typical of mitotic catastrophe in part of the A549 cells. |
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Authors:
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Anna Litwiniec; Alina Grzanka; Anna Helmin-Basa; Lidia Gackowska; Dariusz Grzanka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-07 |
Journal Detail:
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Title: Journal of cancer research and clinical oncology Volume: 136 ISSN: 1432-1335 ISO Abbreviation: J. Cancer Res. Clin. Oncol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-19 Completed Date: 2010-04-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7902060 Medline TA: J Cancer Res Clin Oncol Country: Germany |
Other Details:
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Languages: eng Pagination: 717-36 Citation Subset: IM |
Affiliation:
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Department of Histology and Embryology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru?, Poland. annalitwiniec@wp.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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chemistry,
metabolism* Biological Markers / analysis Cell Aging / drug effects* Cell Death / drug effects* Cell Line, Tumor Doxorubicin / pharmacology* Humans Lung Neoplasms / drug therapy, metabolism, pathology Vimentin / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Biological Markers; 0/Vimentin; 23214-92-8/Doxorubicin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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