| Features of anthocyanin biosynthesis in pap1-D and wild-type Arabidopsis thaliana plants grown in different light intensity and culture media conditions. | |
| | |
MedLine Citation:
|
PMID: 20309578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The number of different anthocyanin molecules potentially produced by Arabidopsis thaliana and which anthocyanin molecule is the first product of anthocyanidin modification remain unknown. To accelerate the understanding of these questions, we investigated anthocyanin biosynthesis in rosette leaves of both pap1-D and wild-type (WT) A. thaliana plants grown in nine growth conditions, which were composed of three light intensities (low light, middle light, and high light) and three media derived from MS medium (medium-1, 2, and 3). These nine growth conditions differentially affected the levels of anthocyanins and pigmentation patterns of rosette leaves, which were closely related to the diversification levels of cyanin structures. The combined growth conditions of high light and either medium-2 or medium-1 induced the most molecular diversity of anthocyanin structures in rosette leaves of pap1-D plants. Twenty cyanin molecules, including five that were previously unknown, were characterized by HPLC-ESI-MS and HPLC-TOF-MS analyses. We detected that the A. thaliana anthocyanin molecule A11 was most likely the first cyanin derived from the multiple modification steps of cyanidin. In addition, in the same growth condition, rosette leaves of pap1-D plants produced much higher levels and more diverse molecular profiling of cyanins than those of WT plants. The transcript levels of PAP1, PAL1, CHS, DFR, and ANS cDNAs were much higher in pap1-D rosette leaves than in WT ones. Furthermore, on the same agar-solidified medium, an enhancement of light intensity increased levels and molecular diversity of cyanins in both pap1-D and WT rosette leaves. In the same light intensity condition, the responses of anthocyanin levels and profiling to medium alternation were different between pap1-D and WT plants. |
| | |
Authors:
|
Ming-Zhu Shi; De-Yu Xie |
Related Documents
:
|
21809098 - Polycyclic aromatic hydrocarbons profiles of spent drilling fluids deposited at emu-uno... 11487688 - Knat2: evidence for a link between knotted-like genes and carpel development. 21755428 - Mutagenicity assessment of contaminated soil in the vicinity of industrial area. 16659468 - Stem sensitivity and ethylene involvement in phototropism of mung bean. 19296388 - Chemical composition of the halophyte plant stachys maritima gouan from the bulgarian b... 23589658 - The life cycle of the antarctic nematode plectus murrayi under laboratory conditions. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-03-23 |
Journal Detail:
|
Title: Planta Volume: 231 ISSN: 1432-2048 ISO Abbreviation: Planta Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-04-14 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
|
Languages: eng Pagination: 1385-400 Citation Subset: IM |
Affiliation:
|
Department of Plant Biology, North Carolina State University, Raleigh, NC 27695, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anthocyanins
/
biosynthesis*,
chemistry,
metabolism Arabidopsis / cytology, drug effects, growth & development*, radiation effects* Biological Transport / drug effects, radiation effects Chromatography, High Pressure Liquid Culture Media / pharmacology* Gene Expression Regulation, Plant / drug effects, radiation effects Genes, Dominant / genetics* Genes, Plant / genetics Glycosylation / drug effects, radiation effects Light* Mass Spectrometry Metabolic Networks and Pathways / drug effects, radiation effects Pigmentation / drug effects, genetics, radiation effects Plant Leaves / drug effects, metabolism, radiation effects RNA, Messenger / genetics, metabolism Transcription Factors / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Anthocyanins; 0/Culture Media; 0/PAP1 protein, Arabidopsis; 0/RNA, Messenger; 0/Transcription Factors; 13306-05-3/cyanidin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Generation of highly pure fusions of colorectal carcinoma and antigen-presenting cells.
Next Document: Monoclonal antibodies to rhamnogalacturonan I backbone.