Document Detail

Features of anthocyanin biosynthesis in pap1-D and wild-type Arabidopsis thaliana plants grown in different light intensity and culture media conditions.
MedLine Citation:
PMID:  20309578     Owner:  NLM     Status:  MEDLINE    
The number of different anthocyanin molecules potentially produced by Arabidopsis thaliana and which anthocyanin molecule is the first product of anthocyanidin modification remain unknown. To accelerate the understanding of these questions, we investigated anthocyanin biosynthesis in rosette leaves of both pap1-D and wild-type (WT) A. thaliana plants grown in nine growth conditions, which were composed of three light intensities (low light, middle light, and high light) and three media derived from MS medium (medium-1, 2, and 3). These nine growth conditions differentially affected the levels of anthocyanins and pigmentation patterns of rosette leaves, which were closely related to the diversification levels of cyanin structures. The combined growth conditions of high light and either medium-2 or medium-1 induced the most molecular diversity of anthocyanin structures in rosette leaves of pap1-D plants. Twenty cyanin molecules, including five that were previously unknown, were characterized by HPLC-ESI-MS and HPLC-TOF-MS analyses. We detected that the A. thaliana anthocyanin molecule A11 was most likely the first cyanin derived from the multiple modification steps of cyanidin. In addition, in the same growth condition, rosette leaves of pap1-D plants produced much higher levels and more diverse molecular profiling of cyanins than those of WT plants. The transcript levels of PAP1, PAL1, CHS, DFR, and ANS cDNAs were much higher in pap1-D rosette leaves than in WT ones. Furthermore, on the same agar-solidified medium, an enhancement of light intensity increased levels and molecular diversity of cyanins in both pap1-D and WT rosette leaves. In the same light intensity condition, the responses of anthocyanin levels and profiling to medium alternation were different between pap1-D and WT plants.
Ming-Zhu Shi; De-Yu Xie
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-03-23
Journal Detail:
Title:  Planta     Volume:  231     ISSN:  1432-2048     ISO Abbreviation:  Planta     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-14     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1385-400     Citation Subset:  IM    
Department of Plant Biology, North Carolina State University, Raleigh, NC 27695, USA.
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MeSH Terms
Anthocyanins / biosynthesis*,  chemistry,  metabolism
Arabidopsis / cytology,  drug effects,  growth & development*,  radiation effects*
Biological Transport / drug effects,  radiation effects
Chromatography, High Pressure Liquid
Culture Media / pharmacology*
Gene Expression Regulation, Plant / drug effects,  radiation effects
Genes, Dominant / genetics*
Genes, Plant / genetics
Glycosylation / drug effects,  radiation effects
Mass Spectrometry
Metabolic Networks and Pathways / drug effects,  radiation effects
Pigmentation / drug effects,  genetics,  radiation effects
Plant Leaves / drug effects,  metabolism,  radiation effects
RNA, Messenger / genetics,  metabolism
Transcription Factors / genetics,  metabolism*
Reg. No./Substance:
0/Anthocyanins; 0/Culture Media; 0/PAP1 protein, Arabidopsis; 0/RNA, Messenger; 0/Transcription Factors; 13306-05-3/cyanidin

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