| Feasibility of using hyperpolarized [1-13C]lactate as a substrate for in vivo metabolic 13C MRSI studies. | |
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MedLine Citation:
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PMID: 18479878 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The development of dynamic nuclear polarization in solution has enabled in vivo 13C MR studies at high signal-to-noise ratio following injection of prepolarized 13C substrates. While prior studies have demonstrated the ability to observe metabolism following injection of hyperpolarized 13C pyruvate, the goal of this study was to develop and test a new hyperpolarized agent for investigating in vivo metabolism, [1-13C]lactate. A preparation for prepolarized 13C lactate and the requisite dissolution media were developed to investigate the feasibility for in vivo 13C MRS/MRSI studies following injection of this hyperpolarized agent. This study demonstrated, for the first time, not only the ability to detect hyperpolarized [1-13C]lactate in vivo but also the metabolic products 13C pyruvate, 13C alanine and 13C bicarbonate following injection in normal rats. The use of 13C lactate as a substrate provided the opportunity to study the conversion of lactate to pyruvate in vivo and to detect the secondary conversions to alanine and bicarbonate through pyruvate. This study also demonstrated the potential value of this hyperpolarized agent to investigate in vivo lactate uptake and metabolism in preclinical animal models. |
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Authors:
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Albert P Chen; John Kurhanewicz; Robert Bok; Duan Xu; David Joun; Vickie Zhang; Sarah J Nelson; Ralph E Hurd; Daniel B Vigneron |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-05-13 |
Journal Detail:
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Title: Magnetic resonance imaging Volume: 26 ISSN: 0730-725X ISO Abbreviation: Magn Reson Imaging Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-01 Completed Date: 2008-12-03 Revised Date: 2011-01-07 |
Medline Journal Info:
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Nlm Unique ID: 8214883 Medline TA: Magn Reson Imaging Country: United States |
Other Details:
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Languages: eng Pagination: 721-6 Citation Subset: IM |
Affiliation:
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Department of Radiology, University of California, Box 2512, San Francisco, CA 94158-2512, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alanine
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metabolism Animals Carbon Isotopes / diagnostic use* Lactic Acid / metabolism* Magnetic Resonance Spectroscopy* Male Pyruvic Acid / metabolism Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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R01 EB007588/EB/NIBIB NIH HHS; R01 EB007588-01/EB/NIBIB NIH HHS; R01 EB007588-02/EB/NIBIB NIH HHS; R21 EB005363/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 127-17-3/Pyruvic Acid; 50-21-5/Lactic Acid; 56-41-7/Alanine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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