Document Detail

Feasibility study of particulate extracellular matrix (P-ECM) and left ventricular assist device (HVAD) therapy in chronic ischemic heart failure bovine model.
MedLine Citation:
PMID:  25423120     Owner:  NLM     Status:  Publisher    
Myocardial recovery with LVAD support is uncommon and unpredictable. We tested the hypothesis that injectable particulate extracellular matrix (P-ECM) with left ventricular assist device (LVAD) support promotes cell proliferation and improves cardiac function. LVAD, P-ECM, and P-ECM+LVAD therapies were investigated in 60-day (n=6) and 90-day (n=6) chronic ischemic heart failure (IHF) calves induced using coronary embolization. P-ECM emulsion (CorMatrix) was injected intramyocardially using a 7-needle pneumatic delivery tool. LVAD (HVAD, HeartWare) were implanted in an LV apex to proximal descending aorta configuration. Cell proliferation was identified using BrdU (5 mg/kg) injections over the last 45 treatment days. Echocardiography was performed weekly. End-organ regional blood flow (RBF) was quantified at study endpoints using fluorescently labeled microspheres. Prior to treatment, IHF calves had an ejection fraction (EF) of 33 ± 2% and LVEDV of 214 ± 18 mL with cardiac cachexia (0.69 ± 0.06 kg/day). Healthy weight gain was restored in all groups (0.89 ± 0.03 kg/day). EF increased with P-ECM+HVAD from 36 ± 5% to 75 ± 2%, HVAD 38 ± 4% to 58 ± 5%, and P-ECM 27 ± 1% to 66 ± 6%. P-ECM+HVAD demonstrated the largest increase in cell proliferation and end-organ RBF. This study demonstrates the feasibility of combined LVAD support with P-ECM injection to stimulate new cell proliferation and improve cardiac function, which warrants further investigation.
Kevin G Soucy; Erin F Smith; Gretel Monreal; Gregg Rokosh; Brad B Keller; Fangping Yuan; Robert G Matheny; Anna M Fallon; Beecher C Lewis; Leslie C Sherwood; Michael A Sobieski; Guruprasad A Giridharan; Steven C Koenig; Mark S Slaughter
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-24
Journal Detail:
Title:  ASAIO journal (American Society for Artificial Internal Organs : 1992)     Volume:  -     ISSN:  1538-943X     ISO Abbreviation:  ASAIO J.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-25     Completed Date:  -     Revised Date:  2014-11-26    
Medline Journal Info:
Nlm Unique ID:  9204109     Medline TA:  ASAIO J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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