Document Detail


Feasibility study: fast liquid chromatography-mass spectrometry for the quantification of aspartic acid in an aspartate drug.
MedLine Citation:
PMID:  14513199     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
We have studied the feasibility of fast high-performance liquid chromatography coupled to electrospray ionization mass spectrometry in the selected ion monitoring mode for the quantitative determination of aspartic acid in an aspartate drug. Internal standardization was required, but mass spectrometric detection allowed for very short retention times of approximately 0.5 min for the analyte and the internal standard without chromatographic separation. The analytical system was found stable, as demonstrated by multiple injections giving a coefficient of variation of 4% for the peak area ratio of aspartic acid and glutamic acid. Calibrations were linear between 0.5 ng and 150 ng aspartic acid injected, with accuracies between 99.8% and 102% found for the back-calculated amounts. Investigation of several drug batches gave reasonable results. Therefore, the method appeared feasible for the determination of aspartic acid in an aspartate drug from 0.3 wt% to 100 wt% aspartic acid.
Authors:
Jochen Schmidt
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Publication Detail:
Type:  Journal Article     Date:  2003-09-25
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  377     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-03     Completed Date:  2004-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1120-3     Citation Subset:  -    
Affiliation:
Preclinical Bioanalytical Chemistry, Solvay Pharmaceuticals, Discovery Hannover, Hans-Böckler-Allee 20, 30173 Hannover, Germany. jochen.schmidt@solvay.com
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