Document Detail

Feasibility study: fast liquid chromatography-mass spectrometry for the quantification of aspartic acid in an aspartate drug.
MedLine Citation:
PMID:  14513199     Owner:  NLM     Status:  PubMed-not-MEDLINE    
We have studied the feasibility of fast high-performance liquid chromatography coupled to electrospray ionization mass spectrometry in the selected ion monitoring mode for the quantitative determination of aspartic acid in an aspartate drug. Internal standardization was required, but mass spectrometric detection allowed for very short retention times of approximately 0.5 min for the analyte and the internal standard without chromatographic separation. The analytical system was found stable, as demonstrated by multiple injections giving a coefficient of variation of 4% for the peak area ratio of aspartic acid and glutamic acid. Calibrations were linear between 0.5 ng and 150 ng aspartic acid injected, with accuracies between 99.8% and 102% found for the back-calculated amounts. Investigation of several drug batches gave reasonable results. Therefore, the method appeared feasible for the determination of aspartic acid in an aspartate drug from 0.3 wt% to 100 wt% aspartic acid.
Jochen Schmidt
Related Documents :
7212929 - Incorporation of 8 succinate per mol nickel into factors f430 by methanobacterium therm...
12788509 - Insulin affects synaptosomal gaba and glutamate transport under oxidative stress condit...
7872839 - A novel pathway of peptide biosynthesis found in methanogenic archaea.
15184939 - Vitreous and retinal amino acid concentrations in experimental central retinal artery o...
11259489 - Nitrogen shuttling between neurons and glial cells during glutamate synthesis.
9275289 - Effect of cysteinesulfinate on fatty acid-dependent uncoupling: modulation of recouplin...
98389 - Immunoreactive secretin in gastrointestinal mucosa of several mammalian species.
18922179 - Essential fatty acids and their metabolites could function as endogenous hmg-coa reduct...
2833499 - Lipid a precursor from pseudomonas aeruginosa is completely acylated prior to addition ...
Publication Detail:
Type:  Journal Article     Date:  2003-09-25
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  377     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-03     Completed Date:  2004-02-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1120-3     Citation Subset:  -    
Preclinical Bioanalytical Chemistry, Solvay Pharmaceuticals, Discovery Hannover, Hans-Böckler-Allee 20, 30173 Hannover, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Rapid immunoanalytical method for the determination of atrazine residues in olive oil.
Next Document:  Non-particulate (continuous bed or monolithic) restricted-access reversed-phase media for sample cle...