Document Detail

Feasibility of fast dynamic MRI for noninvasive monitoring during ectopic liver cell transplantation to the spleen in a porcine model.
MedLine Citation:
PMID:  22623557     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Liver cell transplantation is a promising approach for the treatment of metabolic liver disorders. However, a method for noninvasive monitoring during liver cell transplantation is not available clinically. The aim of this study was to investigate the feasibility of fast dynamic MRI monitoring during liver cell infusion to the spleen, which is considered an ectopic implantation site for liver cell transplantation.
MATERIALS AND METHODS: Porcine liver cells were labeled with micron-sized iron oxide particles and infused to the spleens of pigs (n = 5) via the lineal artery. MRI was performed using a 3-T MR scanner. Initially, T1- and T2-weighted pulse sequences were tested. Thereafter, fast dynamic MRI was performed during cell infusion. MR findings were verified by immunohistological examinations.
RESULTS: Images from static MRI (TR/TE, 2500/105.2) showed significantly lower signal intensity and signal-to-noise ratio after cell infusion compared with pretransplant images. T2-weighted fast dynamic MRI enabled visualization of signal decrease of the spleen during cell infusion. When cells were infused systemically, no signal changes in the spleen were observed.
CONCLUSION: This study shows that fast dynamic MRI can enable noninvasive monitoring during liver cell transplantation to the spleen. This approach could be useful for preclinical studies and for quality control of clinical liver cell transplantation.
Nathanael Raschzok; Jens Pinkernelle; Nils Billecke; Kerstin Nehls; Maciej Powerski; Igor M Sauer; Ulf Teichgräber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  AJR. American journal of roentgenology     Volume:  198     ISSN:  1546-3141     ISO Abbreviation:  AJR Am J Roentgenol     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-24     Completed Date:  2012-08-13     Revised Date:  2013-04-11    
Medline Journal Info:
Nlm Unique ID:  7708173     Medline TA:  AJR Am J Roentgenol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1417-23     Citation Subset:  AIM; IM    
General, Visceral, and Transplantation Surgery, Experimental Surgery and Regenerative Medicine, Charité-Campus Virchow, Universitätsmedizin Berlin, Berlin, Germany.
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MeSH Terms
Cell Culture Techniques
Contrast Media / diagnostic use
Dextrans / diagnostic use
Feasibility Studies
Hepatocytes / transplantation*
In Situ Hybridization, Fluorescence
Magnetic Resonance Imaging / methods*
Magnetite Nanoparticles / diagnostic use
Meglumine / diagnostic use
Models, Animal
Organometallic Compounds / diagnostic use
Reg. No./Substance:
0/Contrast Media; 0/Magnetite Nanoparticles; 0/Organometallic Compounds; 0/gadoterate meglumine; 6284-40-8/Meglumine; 9004-54-0/Dextrans; G6N3J05W84/ferumoxides

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